Effects of ionizing radiation in combination with Erufosine on T98G glioblastoma xenograft tumours: a study in NMRI nu/nu mice

Radiation Oncology

Verena Jendrossek⁵  Wilfried Budach⁶  Claus Belka¹  Michael Bamberg²  Hansjörg Eibl⁴  Lars H Lindner³  Verena Meier²  Guido Henke² 

[1] Department of Radiooncology, University Hospital Grosshadern, Ludwig-Maximilians-University, Marchionistr.15, München, 81377, Germany;Department of Radiooncology, University Hospital Tübingen, Hoppe-Seyler-Str. 3, Tübingen, 72076, Germany;Department of Medicine III, University Hospital Grosshadern, Ludwig-Maximilians-University, Marchionistr.15, München, 81377, Germany;Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen, 37077, Germany;Department of Molecular Cell Biology, Institute of Cell Biology (Cancer Research), University Hospital Essen, Virchowstrasse 173, Essen, 45122, Germany;Department of Radiooncology, University Hospital Düsseldorf, Moorenstrasse 5, Düsseldorf, 40225, Germany

关键词: Local control;    Mouse model;    Xenograft tumours;    T98G glioblastoma;    Combination therapy;    Radiotherapy;    Erufosine;   

Others  :  1155203
DOI  :  10.1186/1748-717X-7-172

 received in 2012-07-12, accepted in 2012-10-13,  发布年份 2012

【 摘 要 】

Background

Erufosine is a promising anticancer drug that increases the efficacy of radiotherapy in glioblastoma cell lines in vitro. Moreover, treatment of nude mice with repeated intraperitoneal or subcutaneous injections of Erufosine is well tolerated and yields drug concentrations in the brain tissue that are higher than the concentrations required for cytotoxic drug effects on glioblastoma cell lines in vitro.

Methods

In the present study we aimed to evaluate the effects of a combined treatment with radiotherapy and Erufosine on growth and local control of T98G subcutaneous glioblastoma xenograft-tumours in NMRI nu/nu mice.

Results

We show that repeated intraperitoneal injections of Erufosine resulted in a significant drug accumulation in T98G xenograft tumours on NMRI nu/nu mice. Moreover, short-term treatment with 5 intraperitoneal Erufosine injections caused a transient decrease in the growth of T98G tumours without radiotherapy. Furthermore, an increased radiation-induced growth delay of T98G xenograft tumours was observed when fractionated irradiation was combined with short-term Erufosine-treatment. However, no beneficial drug effects on fractionated radiotherapy in terms of local tumour control were observed.

Conclusions

We conclude that short-term treatment with Erufosine is not sufficient to significantly improve local control in combination with radiotherapy in T98G glioblastoma xenograft tumours. Further studies are needed to evaluate efficacy of extended drug treatment schedules.

【 授权许可】

   
2012 Henke et al.; licensee BioMed Central Ltd.

【 图 表 】

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