Respiratory Research | |
Food allergy enhances allergic asthma in mice | |
Antoine Magnan2  Marie Bodinier5  Sophie Brouard3  Michel Neunlist1  Christine Sagan4  Guillaume Mahay2  Philippe Aubert1  Marie-Aude Cheminant2  Faouzi Braza2  Camille Rolland-Debord2  David Lair2  Julie Chesné2  Gregory Bouchaud5  Tiphaine Bihouée2  | |
[1] INSERM, UMR U913, Nantes F-44000, France;DHU2020 médecine personnalisée des maladies chroniques, Nantes F-44000, France;INSERM, UMR U1064 and Institut de Transplantation Urologie, Néphrologie (ITUN), Nantes F44093, France;CHU de Nantes, Service d’anatomie et cytologique pathologiques, Nantes, France;INRA, UR1268 BIA, Nantes F-44316, France | |
关键词: Ovalbumin; Mouse; Immunity; House dust mite; Gut; Th2 cytokines; Allergy; Asthma; | |
Others : 1137226 DOI : 10.1186/s12931-014-0142-x |
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received in 2014-09-03, accepted in 2014-11-01, 发布年份 2014 | |
【 摘 要 】
Background
Atopic march refers to the typical transition from a food allergy in early childhood to allergic asthma in older children and adults. However the precise interplay of events involving gut, skin and pulmonary inflammation in this process is not completely understood.
Objectives
To develop a mouse model of mixed food and respiratory allergy mimicking the atopic march and better understand the impact of food allergies on asthma.
Methods
Food allergy to ovalbumin (OVA) was induced through intra-peritoneal sensitization and intra-gastric challenge, and/or a respiratory allergy to house dust mite (HDM) was obtained through percutaneous sensitization and intra-nasal challenges with dermatophagoides farinae (Der f) extract. Digestive, respiratory and systemic parameters were analyzed.
Results
OVA-mediated gut allergy was associated with an increase in jejunum permeability, and a worsening of Der f-induced asthma with stronger airway hyperresponsiveness and pulmonary cell infiltration, notably eosinophils. There was overproduction of the pro-eosinophil chemokine RANTES in broncho-alveolar lavages associated with an enhanced Th2 cytokine secretion and increased total and Der f-specific IgE when the two allergies were present. Both AHR and lung inflammation increased after a second pulmonary challenge.
Conclusion
Gut sensitization to OVA amplifies Der f-induced asthma in mice.
【 授权许可】
2014 Bihouée et al.; licensee BioMed Central Ltd.
【 预 览 】
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