First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua

Particle and Fibre Toxicology

Chengming Wang⁴  Bernhard Kaltenboeck²  Heba S El-Mahallawy³  Jilei Zhang⁴  Kate Ackerson¹  Patrick Kelly¹  Lanjing Wei⁴ 

[1] Ross University School of Veterinary Medicine, Basseterre, St. Kitts and Nevis;Auburn University College of Veterinary Medicine, Auburn, Al, USA;Faculty of Veterinary Medicine, Suez Canal University, Ismailia Egypt;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, P. R. China

关键词: Dirofilaria immitis;    Coxiella burnetii;    Ehrlichia canis;    Anaplasma platys;    Hepatozoon canis;    Babesia gibsoni/vogeli;    Rickettsia felis;    Nicaragua;   

Others  :  807744
DOI  :  10.1186/1756-3305-7-126

 received in 2014-01-26, accepted in 2014-03-10,  发布年份 2014

【 摘 要 】

Background

Although many vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas and potential zoonoses, there is little information on these conditions in Central America.

Methods

Seven qPCRs for vector-borne pathogens were performed on a Roche LightCycler PCR Instrument to investigate their prevalence in a convenience sample of whole blood samples from apparently healthy dogs in Nicaragua. Also, a qPCR targeting the canine hydroxymethylbilane synthase (HMBS) gene was used as an endogenous internal control and verified the quality and quantity of DNA in the samples was appropriate for the study.

Results

We found DNA of Rickettsia felis (5%), Babesia spp. (26%), Hepatozoon canis (51%), Anaplasma platys (13%) and Ehrlichia canis (56%) in the 39 dogs studied. The qPCRs for Coxiella burnetii and Dirofilaria immitis were negative. Of the 30 (80%) dogs that were positive by qPCR, 12 (31%) were positive for one agent, 11 (28%) for two, 3 (8%) for three, and 4 (10%) for four agents.

Conclusions

This is the first report of B. gibsoni in dogs from Central America and the first recording of vector-borne agents in dogs from Nicaragua. Dogs in Nicaragua are commonly infected with a variety of vector-borne pathogens, some of which may also infect people.

【 授权许可】

   
2014 Wei et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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