Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms

Veterinary Research

Geertrui Rasschaert⁴  Marc Heyndrickx²  Lieve Herman⁴  Patrick Butaye³  Freddy Haesebrouck²  Larissa J Pletinckx¹  Florence Crombé³  Marijke Verhegghe² 

[1] Department Biosystems, Division Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, Heverlee, 3001, Belgium;Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, Merelbeke, 9820, Belgium;Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre (VAR), Groeselenberg 99, Brussels, 1180, Belgium;Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Food safety research group, Brusselsesteenweg 370, Melle, 9090, Belgium

关键词: Genetic diversity;    Pigs;    Epidemiology;    Molecular typing;    LA-MRSA;   

Others  :  1147012
DOI  :  10.1186/s13567-014-0089-4

 received in 2013-07-02, accepted in 2014-08-08,  发布年份 2014

【 摘 要 】

During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrophoresis (PFGE), spa typing, and SCCmec typing to study the genetic diversity of LA-MRSA isolates and to determine possible MRSA sources for pig(let)s. PFGE, spa typing, and SCCmec typing revealed the presence of one or few dominant genotype(s) per farm. In contrast, 212 MLVA types were detected on the four farms, forming one cluster on farm A, three on farm B, four on farm C and two on farm D. The genotype, found on farm A was unique for this farm. Farms B, C and D shared one cluster. In general, MLVA types from these clusters were isolated from piglets, sows, and the environment on various sampling events. Piglets carried MLVA types both related and unrelated to their mother sows¿ MLVA types at farrowing and onwards. In conclusion, molecular typing revealed that within a farm one or a few dominant strain(s) are widespread. Potential MRSA sources for piglets were mother sows, the environment and other piglets.

【 授权许可】

   
2014 Verhegghe et al.; licensee BioMed Central Ltd.

【 图 表 】

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