Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting

Molecular Cytogenetics

Vladimir A. Trifonov³  Alexander S. Graphodatsky¹  Nadezhda V. Vorobieva¹  Marina A. Korentovich²  Elena A. Interesova⁴  Natalia A. Lemskaya³  Olga L. Gladkikh³  Violetta R. Beklemisheva³  Anastasia I. Kulemzina³  Natalya A. Serdyukova³  Larisa S. Biltueva³  Svetlana A. Romanenko¹ 

[1] Novosibirsk State University, Novosibirsk, Russia;Federal State Budgetary Scientific Institution “State Scientific-and-Production Centre for Fisheries (Gosrybcenter)”, Tyumen, Russia;Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia;Tomsk State University, Tomsk, Russia

关键词: Microdissection;    FISH;    rRNA;    Telomeric repeat;    Satellite DNA;    Banding;    Fish cell line;    Acipenseriformes;   

Others  :  1233886
DOI  :  10.1186/s13039-015-0194-8

 received in 2015-09-04, accepted in 2015-11-07,  发布年份 2015

【 摘 要 】

Background

Acipenseriformes take a basal position among Actinopteri and demonstrate a striking ploidy variation among species. The sterlet (Acipenser ruthenus, Linnaeus, 1758; ARUT) is a diploid 120-chromosomal sturgeon distributed in Eurasian rivers from Danube to Enisey. Despite a high commercial value and a rapid population decline in the wild, many genomic characteristics of sterlet (as well as many other sturgeon species) have not been studied.

Results

Cell lines from different tissues of 12 sterlet specimens from Siberian populations were established following an optimized protocol. Conventional cytogenetic studies supplemented with molecular cytogenetic investigations on obtained fibroblast cell lines allowed a detailed description of sterlet karyotype and a precise localization of 18S/28S and 5S ribosomal clusters. Localization of sturgeon specific HindIII repetitive elements revealed an increased concentration in the pericentromeric region of the acrocentric ARUT14, while the total sterlet repetitive DNA fraction (C ₀ t30) produced bright signals on subtelomeric segments of small chromosomal elements. Chromosome and region specific probes ARUT1p, 5, 6, 7, 8 as well as 14 anonymous small sized chromosomes (probes A-N) generated by microdissection were applied in chromosome painting experiments. According to hybridization patterns all painting probes were classified into two major groups: the first group (ARUT5, 6, 8 as well as microchromosome specific probes C, E, F, G, H, and I) painted only a single region each on sterlet metaphases, while probes of the second group (ARUT1p, 7 as well as microchromosome derived probes A, B, D, J, K, M, and N) marked two genomic segments each on different chromosomes. Similar results were obtained on male and female metaphases.

Conclusions

The sterlet genome represents a complex mosaic structure and consists of diploid and tetraploid chromosome segments. This may be regarded as a transition stage from paleotetraploid (functional diploid) to diploid genome condition. Molecular cytogenetic and genomic studies of other 120- and 240-chromosomal sturgeons are needed to reconstruct genome evolution of this vertebrate group.

【 授权许可】

   
2015 Romanenko et al.

【 图 表 】

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