An optical imaging chamber for viewing living plant cells and tissues at high resolution for extended periods

Plant Methods

Peter Shaw¹  Jordi Chan¹  Chris Hindle¹  Grant Calder¹ 

[1] Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK

关键词: Plant tissue;    Live cell imaging;    Perfusion;    Time-lapse microscopy;    Imaging chamber;    Arabidopsis;   

Others  :  1174968
DOI  :  10.1186/s13007-015-0065-7

 received in 2015-02-16, accepted in 2015-03-12,  发布年份 2015

【 摘 要 】

Background

Recent developments in both microscopy and fluorescent protein technologies have made live imaging a powerful tool for the study of plant cells. However, the complications of keeping plant material alive during a long duration experiment while maintaining maximum resolution has limited the use of these methods.

Results

Here, we describe an imaging chamber designed to overcome these limitations, which is flexible enough to support a range of sizes of plant materials. We were able use confocal microscopy to follow growth and development of plant cells and tissues over several days. The chamber design is based on a perfusion system, so that the addition of drugs and other experimental treatments are also possible.

Conclusions

In this article we present a design of imaging chamber that makes it possible to image plant material with high resolution for extended periods of time.

【 授权许可】

   
2015 Calder et al.; licensee BioMed Central.

【 图 表 】

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