Evidence for natural recombination between mink enteritis virus and canine parvovirus

Virology Journal

Hua Wu¹  Xijun Yan¹  Hang Zhao¹  Hongli Xu¹  Shen Yang¹  Yuening Cheng¹  Li Yi¹  Shipeng Cheng¹  Jianke Wang¹ 

[1] State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China

关键词: Recombination;    Mink enteritis virus;    Parvovirus;   

Others  :  1153366
DOI  :  10.1186/1743-422X-9-252

 received in 2012-07-02, accepted in 2012-10-18,  发布年份 2012

【 摘 要 】

A virus was isolated from mink showing clinical and pathological signs of enteritis in China. This virus, designated MEV/LN-10, was identified as mink enteritis virus (MEV) based on its cytopathic effect in the feline F81 cell line, the hemagglutination (HA) and hemagglutination inhibition (HI) assay, electron microscopy (EM) and animal infection experiments. The complete viral genome was cloned and sequenced. Phylogenetic and recombination analyses on the complete MEV/LN-10 genome showed evidence of recombination between MEV and canine parvovirus (CPV). The genome was composed of the NS1 gene originating from CPV while the VP1 gene was of MEV origin. This is the first demonstration of recombination between a CPV and MEV in nature. Our findings not only provide valuable evidence indicating that recombination is an important genetic mechanism contributing to the variation and evolution of MEV, but also that heterogeneous recombination can occur in the feline parvovirus subspecies.

【 授权许可】

   
2012 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Uttenthal A, Larsen S, Lund E, Bloom ME, Storgard T, Alexandersen S: Analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology. J Virol 1990, 64:2768-2779.
• [2]Schofield FW: Virus enteritis in mink. N Am Vet 1949, 30:651-654.
• [3]Wills CG: Notes on infectious enteritis of mink and its relationship to feline enteritis. Can J Comp Med Vet Sci 1952, 16:419-420.
• [4]Steinel A, Parrish CR, Bloom ME, Truyen U: Parvovirus infections in wild carnivores. J Wildl Dis 2001, 37:594-607.
• [5]Decaro N, Buonavoglia C: Canine parvovirus-A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 2012, 155:1-12.
• [6]Langeveld JP, Kamstrup S, Uttenthal A, Strandbygaard B, Vela C, Dalsgaard K, Beekman NJ, Meloen RH, Casal JI: Full protection in mink against mink enteritis virus with new generation canine parvovirus vaccines based on synthetic peptide or recombinant protein. Vaccine 1995, 13:1033-1037.
• [7]Zhao X, Yin Z, Zhao Y, Li Z, Wu X: Nucleotide sequence and genome structure of mink enteritis virus. Yi Chuan Xue Bao 1993, 20:279-284.
• [8]Kariatsumari T, Horiuchi M, Hama E, Yaguchi K, Ishigurio N, Goto H, Shinagawa M: Construction and nucleotide sequence analysis of an infectious DNA clone of the autonomous parvovirus, mink enteritis virus. J Gen Virol 1991, 72(Pt 4):867-875.
• [9]Mochizuki M, Ohshima T, Une Y, Yachi A: Recombination between vaccine and field strains of canine parvovirus is revealed by isolation of virus in canine and feline cell cultures. J Vet Med Sci 2008, 70:1305-1314.
• [10]Ohshima T, Mochizuki M: Evidence for recombination between feline panleukopenia virus and canine parvovirus type 2. J Vet Med Sci 2009, 71:403-408.
• [11]Zhang R, Yang S, Zhang W, Zhang T, Xie Z, Feng H, Wang S, Xia X: Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China. Virus genes 2010, 40:397-402.
• [12]Yan XJ, Chai XL, Wu W, Wang FX, Shao XQ, Zhao CF, Jiang LL: Isolation and identification of a mink enteritis parvovirus strain. Anim Husbandry Vet Med 2007, 39:25-26.
• [13]Krunajevic T: Experimental virus enteritis in mink. A pathologic-anatomical and electron microscopical study. Acta veterinaria Scandinavica Supplementum 1970, 30(Suppl 30):31-88.
• [14]Chen T, Zhao JJ, Zhang HL, Wu W, Qian AD, Yan XJ: Cloning and sequence analysis of complete genome of mink enteritis virus B strain. Journal of Jilin Agricultural University 2010, 32:81-85.
• [15]Boni MF, Posada D, Feldman MW: An exact nonparametric method for inferring mosaic structure in sequence triplets. Genetics 2007, 176:1035-1047.
• [16]Gibbs MJ, Armstrong JS, Gibbs AJ: Sister-scanning: a Monte Carlo procedure for assessing signals in recombinant sequences. Bioinformatics (Oxford, England) 2000, 16:573-582.
• [17]Kosakovsky Pond SL, Posada D, Gravenor MB, Woelk CH, Frost SD: Automated phylogenetic detection of recombination using a genetic algorithm. Mol Biol Evol 2006, 23:1891-1901.
• [18]Martin D, Rybicki E: RDP: detection of recombination amongst aligned sequences. Bioinformatics (Oxford, England) 2000, 16:562-563.
• [19]Martin DP, Posada D, Crandall KA, Williamson C: A modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints. AIDS research and human retroviruses 2005, 21:98-102.
• [20]Padidam M, Sawyer S, Fauquet CM: Possible emergence of new geminiviruses by frequent recombination. Virology 1999, 265:218-225.
• [21]Posada D, Crandall KA: Evaluation of methods for detecting recombination from DNA sequences: computer simulations. Proc Natl Acad Sci U S A 2001, 98:13757-13762.
• [22]Smith JM: Analyzing the mosaic structure of genes. J Mol Evol 1992, 34:126-129.
• [23]Shackelton LA, Hoelzer K, Parrish CR, Holmes EC: Comparative analysis reveals frequent recombination in the parvoviruses. J Gen Virol 2007, 88:3294-3301.
• [24]Parrish CR: Host range relationships and the evolution of canine parvovirus. Vet Microbiol 1999, 69:29-40.
• [25]Stucker KM, Pagan I, Cifuente JO, Kaelber JT, Lillie TD, Hafenstein S, Holmes EC, Parrish CR: The role of evolutionary intermediates in the host adaptation of canine parvovirus. J Virol 2012, 86:1514-1521.
• [26]Allison AB, Harbison CE, Pagan I, Stucker KM, Kaelber JT, Brown JD, Ruder MG, Keel MK, Dubovi EJ, Holmes EC, Parrish CR: Role of multiple hosts in the cross-species transmission and emergence of a pandemic parvovirus. J Virol 2012, 86:865-872.
• [27]Clegg SR, Coyne KP, Parker J, Dawson S, Godsall SA, Pinchbeck G, Cripps PJ, Gaskell RM, Radford AD: Molecular epidemiology and phylogeny reveal complex spatial dynamics in areas where canine parvovirus is endemic. J Virol 2011, 85:7892-7899.
• [28]Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, Elia G, Zicola A, Davis C, Thompson G, Thiry E, et al.: The study molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis 2007, 13:1222-1224.
• [29]Parrish CR, Leathers CW, Pearson R, Gorham JR: Comparisons of feline panleukopenia virus, canine parvovirus, raccoon parvovirus, and mink enteritis virus and their pathogenicity for mink and ferrets. Am J Vet Res 1987, 48:1429-1435.
• [30]Joao Vieira M, Silva E, Desario C, Decaro N, Carvalheira J, Buonavoglia C, Thompson G: Natural co-infection with 2 parvovirus variants in dog. Emerg Infect Dis 2008, 14:678-679.
• [31]Battilani M, Gallina L, Vaccari F, Morganti L: Co-infection with multiple variants of canine parvovirus type 2 (CPV-2). Vet Res Commun 2007, 31(Suppl 1):209-212.
• [32]Battilani M, Balboni A, Ustulin M, Giunti M, Scagliarini A, Prosperi S: Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats. Vet Res 2011, 42:43. BioMed Central Full Text