Proteome Science | |
Nuclear proteome response to cell wall removal in rice (Oryza sativa) | |
Zhaohua Peng2  Ken Pendarvis1  Babi Ramesh Reddy Nallamilli2  Jian Zhang2  Feng Tan2  Hana Mujahid2  | |
[1] Present Address: College of Agriculture and Life Sciences, University of Arizona, P.O. Box 210036, Tucson, AZ 85721, USA;Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA | |
关键词: Comparative proteomics; Cell wall; Nuclear proteins; Rice; Protoplast; | |
Others : 816882 DOI : 10.1186/1477-5956-11-26 |
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received in 2013-02-05, accepted in 2013-06-13, 发布年份 2013 | |
【 摘 要 】
Plant cells are routinely exposed to various pathogens and environmental stresses that cause cell wall perturbations. Little is known of the mechanisms that plant cells use to sense these disturbances and transduce corresponding signals to regulate cellular responses to maintain cell wall integrity. Previous studies in rice have shown that removal of the cell wall leads to substantial chromatin reorganization and histone modification changes concomitant with cell wall re-synthesis. But the genes and proteins that regulate these cellular responses are still largely unknown. Here we present an examination of the nuclear proteome differential expression in response to removal of the cell wall in rice suspension cells using multiple nuclear proteome extraction methods. A total of 382 nuclear proteins were identified with two or more peptides, including 26 transcription factors. Upon removal of the cell wall, 142 nuclear proteins were up regulated and 112 were down regulated. The differentially expressed proteins included transcription factors, histones, histone domain containing proteins, and histone modification enzymes. Gene ontology analysis of the differentially expressed proteins indicates that chromatin & nucleosome assembly, protein-DNA complex assembly, and DNA packaging are tightly associated with cell wall removal. Our results indicate that removal of the cell wall imposes a tremendous challenge to the cells. Consequently, plant cells respond to the removal of the cell wall in the nucleus at every level of the regulatory hierarchy.
【 授权许可】
2013 Mujahid et al.; licensee BioMed Central Ltd.
【 预 览 】
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