Distinct variation in vector competence among nine field populations of Aedes aegypti from a Brazilian dengue-endemic risk city

Particle and Fibre Toxicology

Paulo FP Pimenta⁴  Douglas E Norris⁵  Nágila FC Secundino⁴  Jorge R Arias¹  José EM Pessanha²  Luciana D Silva⁶  Breno M Silva³  Bárbara A Chaves⁴  Juliana MT Bezerra⁴  Fabrício F Melo⁴  Caroline M Gonçalves⁴ 

[1] Medical Entomologist, Retired Consultant, Manassas, Virginia, USA;Municipal Health Secretariat of Belo Horizonte, Belo Horizonte, Minas Gerais, Brazil;Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil;Laboratory of Medical Entomology, René Rachou Research Centre- FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil;Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA;Department of Internal Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

关键词: Disseminated infection rate;    Vector competence;    Infection rate;    Field population;    Aedes aegypti;    Dengue virus (DENV);   

Others  :  1183559
DOI  :  10.1186/1756-3305-7-320

 received in 2014-04-29, accepted in 2014-06-28,  发布年份 2014

【 摘 要 】

Background

In Brazil, dengue epidemics erupt sporadically throughout the country and it is unclear if outbreaks may initiate a sustainable transmission cycle. There are few studies evaluating the ability of Brazilian Aedes aegypti populations to transmit dengue virus (DENV). The aim of this study was to compare DENV susceptibility of field-captured Ae. aegypti populations from nine distinct geographic areas of the city of Belo Horizonte in 2009 and 2011. Infection Rate (IR), Vector Competence (VC) and Disseminated Infection Rate (DIR) were determined.

Methods

Aedes aegypti eggs from each region were collected and reared separately in an insectary. Adult females were experimentally infected with DENV-2 and the virus was detected by qPCR in body and head samples. Data were analyzed with the Statistical Package for the Social Sciences version 17.

Results

IR varied from 40.0% to 82.5% in 2009 and 60.0% to 100.0% in 2011. VC ranged from 25.0% to 77.5% in 2009 and 25.0% to 80.0% in 2011. DIR oscillated from 68.7% to 100.0% in 2009 and 38.4% to 86.8 in 2011. When the results were evaluated by a logistic model using IR as covariate, North, Barreiro, South-Central and Venda Nova showed the strongest association in 2009. In 2011, a similar association was observed for South-Central, Venda Nova, West and Northeast regions. Using VC as covariate, South-Central and Venda Nova showed the most relevant association in 2009. In 2011, South-Central, Venda Nova and Barreiro presented the greatest revelation associations. When DIR data were analyzed by logistic regression models, Pampulha, South-Central, Venda Nova, West, Northeast and East (2009) as well as South-Central, Venda Nova and West (2011) were the districts showing the strongest associations.

Conclusions

We conclude that Ae. aegypti populations from Belo Horizonte exhibit wide variation in vector competence to transmit dengue. Therefore, vector control strategies should be adapted to the available data for each region. Further analysis should be conducted to better understand the reasons for this large variability in vector competence and how these parameters correlate with epidemiological findings in subsequent years.

【 授权许可】

   
2014 Gonçalves et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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