First introduction of pandemic influenza A/H1N1 and detection of respiratory viruses in pediatric patients in Central African Republic

Virology Journal

Mirdad Kazanji¹  Philippe Buchy³  Kathleen Victoir⁵  Jean Chrysostome Gody⁶  Benjamin Selekon²  Francis Komoyo²  Alexandre Manirakiza⁴  Vianney Tricou¹  Emmanuel Nakouné¹ 

[1] Virology department, Institut Pasteur de Bangui, Bangui, Central African Republic;National Influenza Centre, Institut Pasteur de Bangui, Bangui, Central African Republic;Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia;Epidemiology Unit, Institut Pasteur de Bangui, Bangui, Central African Republic;International Division, Institut Pasteur, Paris, France;Complexe Pédiatrique de Bangui, Bangui, Central African Republic

关键词: Pediatric patients;    Parainfluenza virus;    Respiratory syncytial virus;    Influenza B;    Pandemic influenza A/H1N1 2009;    Acute respiratory illness;    Molecular diagnosis;   

Others  :  1151835
DOI  :  10.1186/1743-422X-10-49

 received in 2012-09-20, accepted in 2013-02-06,  发布年份 2013

【 摘 要 】

Background

Acute viral respiratory illnesses in children in sub-Saharan Africa have received relatively little attention, although they are much more frequent causes of morbidity and mortality than in developed countries. Active surveillance is essential to identify the causative agents and to improve clinical management, especially in the context of possible circulation of pandemic viruses.

Findings

A prospective study was conducted in the Central African Republic (CAR) between January and December 2010 among infants and children aged 0–15 years attending sentinel sites for influenza-like illness or acute respiratory illness. Nasopharyngeal swabs were collected, and one-step real-time and multiplex reverse transcription-polymerase chain reaction were used to detect respiratory viruses. Respiratory viruses were detected in 49 of the 329 (14.9%) nasopharyngeal samples: 29 (8.8%) contained influenza viruses (5 (1.5%) had pandemic influenza A/H1N1 virus and 24 (7.3%) had influenza B viruses), 11 (3.3%) contained parainfluenza viruses types 1 and 3 and 9 (2.7%) contained human respiratory syncytial virus. Most cases were detected during the rainy season in the CAR. Analysis of the amplicon sequences confirmed the identity of each detected virus.

Conclusions

The influenza surveillance system in the CAR has provided valuable data on the seasonality of influenza and the circulation of other respiratory viruses. Our network could therefore play a valuable role in the prevention and control of influenza epidemics in the CAR.

【 授权许可】

   
2013 Nakouné et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Scott JA, English M: What are the implications for childhood pneumonia of successfully introducing Hib and pneumococcal vaccines in developing countries? PLoS Med 2008, 5(4):e86.
• [2]Zar HJ, Madhi SA: Childhood pneumonia–progress and challenges. S Afr Med J 2006, 96(9 Pt 2):890-900.
• [3]Fabbiani M, Terrosi C, Martorelli B, Valentini M, Bernini L, Cellesi C, Cusi MG: Epidemiological and clinical study of viral respiratory tract infections in children from Italy. J Med Virol 2009, 81(4):750-756.
• [4]Monto AS: Occurrence of respiratory virus: time, place and person. Pediatr Infect Dis J 2004, 23(1 Suppl):S58-64.
• [5]Tregoning JS, Schwarze J: Respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology. Clin Microbiol Rev 2010, 23(1):74-98.
• [6]Yazdanbakhsh M, Kremsner PG: Influenza in Africa. PLoS Med 2009, 6(12):e1000182.
• [7]Dosseh A, Ndiaye K, Spiegel A, Sagna M, Mathiot C: Epidemiological and virological influenza survey in Dakar, Senegal: 1996–1998. Am J Trop Med Hyg 2000, 62(5):639-643.
• [8]Nicholson KG, Wood JM, Zambon M: Influenza. Lancet 2003, 362(9397):1733-1745.
• [9]Duchamp MB, Casalegno JS, Gillet Y, Frobert E, Bernard E, Escuret V, Billaud G, Valette M, Javouhey E, Lina B, et al.: Pandemic A(H1N1)2009 influenza virus detection by real time RT-PCR: is viral quantification useful? Clin Microbiol Infect 2010, 16(4):317-321.
• [10]Bellau-Pujol S, Vabret A, Legrand L, Dina J, Gouarin S, Petitjean-Lecherbonnier J, Pozzetto B, Ginevra C, Freymuth F: Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Meth 2005, 126(1–2):53-63.
• [11]Buecher C, Mardy S, Wang W, Duong V, Vong S, Naughtin M, Vabret A, Freymuth F, Deubel V, Buchy P: Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons. J Med Virol 2010, 82(10):1762-1772.
• [12]Archer B, Cohen C, Naidoo D, Thomas J, Makunga C, Blumberg L, Venter M, Timothy G, Puren A, McAnerney J, et al.: Interim report on pandemic H1N1 influenza virus infections in South Africa, April to October 2009: epidemiology and factors associated with fatal cases. Euro Surveill 2009, 14(42):pii19369.
• [13]CDC: Introduction and transmission of 2009 pandemic influenza A (H1N1) virus--Kenya, June-July 2009. Morb Mortal Wkly Rep 2009, 58(41):1143-1146.
• [14]Wane J, Nyatanyi T, Nkunda R, Rukelibuga J, Ahmed Z, Biedron C, Kabeja A, Muhimpundu MA, Kabanda A, Antara S, et al.: 2009 pandemic influenza A (H1N1) virus outbreak and response–Rwanda, October, 2009-May, 2010. PLoS One 2012, 7(6):e31572.
• [15]Baker M, Kelly H, Wilson N: Pandemic H1N1 influenza lessons from the southern hemisphere. Eur Surveill 2009, 14(42):pii19370.
• [16]Opatowski L, Fraser C, Griffin J, de Silva E, Van Kerkhove MD, Lyons EJ, Cauchemez S, Ferguson NM: Transmission characteristics of the 2009 H1N1 influenza pandemic: comparison of 8 Southern hemisphere countries. PLoS Pathol 2011, 7(9):e1002225.
• [17]Kadjo HA, Ekaza E, Coulibaly D, Kouassi DP, Nzussouo NT, Kouakou B, Ouattara A, Adjogoua EV, Akoua-Koffi CG, Elia GA, et al.: Sentinel surveillance for influenza and other respiratory viruses in Côte d’Ivoire, 2003–2010. Influenza Other Resp Viruses
• [18]Berkley JA, Munywoki P, Ngama M, Kazungu S, Abwao J, Bett A, Lassauniére R, Kresfelder T, Cane PA, Venter M, et al.: Viral etiology of severe pneumonia among Kenyan infants and children. JAMA 2010, 303(20):2051-2057.
• [19]Kwofie TB, Anane YA, Nkrumah B, Annan A, Nguah SB, Owusu M: Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana. Virol J 2012, 9:78. BioMed Central Full Text
• [20]Neuzil KM, Zhu Y, Griffin MR, Edwards KM, Thompson JM, Tollefson SJ, Wright PF: Burden of interpandemic influenza in children younger than 5 years: a 25-year prospective study. J Infect Dis 2002, 185(2):147-152.
• [21]Iwane MK, Edwards KM, Szilagyi PG, Walker FJ, Griffin MR, Weinberg GA, Coulen C, Poehling KA, Shone LP, Balter S, et al.: Population-based surveillance for hospitalizations associated with respiratory syncytial virus, influenza virus, and parainfluenza viruses among young children. Pediatrics 2004, 113(6):1758-1764.
• [22]Pecchini R, Berezin EN, Felício MC, Passos SD, Souza MC, Lima LR, Ueda M, Matsumoto TK, Durigon EL: Incidence and clinical characteristics of the infection by the respiratory syncytial virus in children admitted in Santa Casa de São Paulo Hospital. Braz J Infect Dis 2008, 12(6):476-479.
• [23]Chun JK, Lee JH, Kim HS, Cheong HM, Kim KS, Kang C, Kim DS: Establishing a surveillance network for severe lower respiratory tract infections in Korean infants and young children. Eur J Clin Microbiol Infect Dis 2009, 28(7):841-844.
• [24]Pierangeli A, Scagnolari C, Selvaggi C, Monteleone K, Verzaro S, Nenna R, Cangiano G, Moretti C, Papoff P, Antonelli G, et al.: Virological and clinical characterization of respiratory infections in children attending an emergency department during the first autumn-winter circulation of pandemic A (H1N1) 2009 influenza virus. Clin Microbiol Infect 2012, 18(4):366-373.
• [25]Wang W, Cavailler P, Ren P, Zhang J, Dong W, Yan H, Mardy S, Cailhol J, Buchy P, Sheng J, et al.: Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai. J Clin Virol 2010, 49(3):211-218.
• [26]Brittain-Long R, Andersson LM, Olofsson S, Lindh M, Westin J: Seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay. Scand J Infect Dis 2012, 44(1):9-17.
• [27]Centers for Disease Control and Prevention: Antibiotic resistance among nasopharyngeal isolates of Streptococcus pneumoniae and Haemophilus influenzae--Bangui, Central African Republic, 1995. Morb Mortal Wkly Rep 1997, 46(3):62-64.
• [28]Mastro TD, Nomani NK, Ishaq Z, Ghafoor A, Shaukat NF, Esko E, Leinonen M, Henrichsen J, Breiman RF, Schwartz B: Use of nasopharyngeal isolates of Streptococcus pneumoniae and Haemophilus influenzae from children in Pakistan for surveillance for antimicrobial resistance. Pediatr Infect Dis J 1993, 12(10):824-830.
• [29]Cutts FT, Zaman SM, Enwere G, Jaffar S, Levine OS, Okoko JB, Oluwalana C, Vaughan A, Obaro SK, Leach A, et al.: Efficacy of nine-valent pneumococcal conjugate vaccine against pneumonia and invasive pneumococcal disease in The Gambia: randomised, double-blind, placebo-controlled trial. Lancet 2005, 365(9465):1139-1146.
• [30]Mulholland K, Hilton S, Adegbola R, Usen S, Oparaugo A, Omosigho C, Weber M, Palmer A, Schneider G, Jobe K, et al.: Randomised trial of Haemophilus influenzae type-b tetanus protein conjugate vaccine [corrected] for prevention of pneumonia and meningitis in Gambian infants. Lancet 1997, 349(9060):1191-1197.
• [31]Steffen C, Debellut F, Gessner BD, Kasolo FC, Yahaya AA, Ayebazibwe N, Bassong O, Cardoso Y, Kebede S, Manoncourt S, et al.: Improving influenza surveillance in sub-Saharan Africa. Bull World Health Organ 2012, 90(4):301-305.
• [32]Barakat A, Benjouad A, Manuguerra JC, El Aouad R, Van der Werf S: Virological surveillance in Africa can contribute to early detection of new genetic and antigenic lineages of influenza viruses. J Infect Dev Countries 2011, 5(4):270-277.
• [33]Ezzati M, Lopez AD, Rodgers A, Vander Hoorn S, Murray CJ: Selected major risk factors and global and regional burden of disease. Lancet 2002, 360:1347-1360.
• [34]Lopez AD, Mathers CD, Ezzati M, Jamison DT, Murray CJ: Global and regional burden of disease and risk factors 2001: systematic analysis of population health data. Lancet 2006, 367:1747-1757.