Retinoic acid regulates olfactory progenitor cell fate and differentiation

Neural Development

Raj K Ladher²  Pascal Dollé¹  Valérie Fraulob¹  Monika Rataj-Baniowska¹  Siu-Shan Mak²  Yoko Matsuoka²  Yuko Muta²  Laura Cammas¹  Marie Paschaki¹ 

[1] Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104 CNRS, U 964 INSERM, Université de Strasbourg, B.P. 10142, 67404, Illkirch Cedex, France;Laboratory for Sensory Development, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan

关键词: Stem cells;    Sensory systems;    Retinoid signaling;    RALDH;    Neuronal differentiation;    Olfactory neurons;   

Others  :  804504
DOI  :  10.1186/1749-8104-8-13

 received in 2013-02-06, accepted in 2013-05-29,  发布年份 2013

【 摘 要 】

Background

In order to fulfill their chemosensory function, olfactory neurons are in direct contact with the external environment and are therefore exposed to environmental aggressive factors. Olfaction is maintained through life because, unlike for other sensory neuroepithelia, olfactory neurons have a unique capacity to regenerate after trauma. The mechanisms that control the ontogenesis and regenerative ability of these neurons are not fully understood. Here, we used various experimental approaches in two model systems (chick and mouse) to assess the contribution of retinoic acid signaling in the induction of the olfactory epithelium, the generation and maintenance of progenitor populations, and the ontogenesis and differentiation of olfactory neurons.

Results

We show that retinoic acid signaling, although dispensable for initial induction of the olfactory placode, plays a key role in neurogenesis within this neuroepithelium. Retinoic acid depletion in the olfactory epithelium, both in chick and mouse models, results in a failure of progenitor cell maintenance and, consequently, differentiation of olfactory neurons is not sustained. Using an explant system, we further show that renewal of olfactory neurons is hindered if the olfactory epithelium is unable to synthesize retinoic acid.

Conclusions

Our data show that retinoic acid is not a simple placodal inductive signal, but rather controls olfactory neuronal production by regulating the fate of olfactory progenitor cells. Retinaldehyde dehydrogenase 3 (RALDH3) is the key enzyme required to generate retinoic acid within the olfactory epithelium.

【 授权许可】

   
2013 Paschaki et al.; licensee BioMed Central Ltd.

【 图 表 】

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