Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine astrocytes

Virology Journal

Kangsheng Li¹  Zhiwu Jiang¹  Gang Xin¹  Xiaoxuan Chen¹  Dangui Zhang²  Weizhong Li¹  Gefei Wang¹  Jun Zeng¹ 

[1] Key laboratory of infectious diseases and molecular immunopathology of Guangdong province, Department of Microbiology & Immunology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, P.R. China;Research Center of Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou 515065, P.R. China

关键词: Neuroinflammation;    Cytokine;    Cell death;    Astrocyte;    Coxsackievirus;    Viral infection;   

Others  :  1150175
DOI  :  10.1186/1743-422X-10-157

 received in 2012-11-13, accepted in 2013-05-20,  发布年份 2013

【 摘 要 】

Background

Coxsackievirus commonly infects children and occasionally causes severe meningitis and/or encephalitis in the newborn. The underlying mechanism(s) behind the central nervous system pathology is poorly defined.

Methods

It is hypothesized that astrocytes may be involved in inflammatory response induced by CVB3 infection. Here we discuss this hypothesis in the context of CVB3 infection and associated inflammatory response in primary mouse astrocytes.

Results

The results showed that coxsackievirus receptor (CAR) was distributed homogeneously on the astrocytes, and that CVB3 could infect and replicate in astrocytes, with release of infectious virus particles. CVB3 induced cytopathic effect and production of proinflammatory cytokines IL-1β, TNF-α, IL-6, and chemokine CXCL10 from astrocytes.

Conclusion

These data suggest that direct astrocyte damage and cytokines induction could be a mechanism of virus-induced meningitis and/or encephalitis.

【 授权许可】

   
2013 Zeng et al.; licensee BioMed Central Ltd.

【 图 表 】

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