Molecular evolution of the H6 subtype influenza a viruses from poultry in eastern China from 2002 to 2010

Virology Journal

Xiufan Liu¹  Xiaoquan Wang¹  Shunlin Hu¹  Daxin Peng¹  Zhiqiang Duan¹  Quangang Xu¹  Jinjin Pan¹  Qingqing Song¹  Kunkun Zhao¹  Xiaobing Gu¹  Xinlun Lu¹  Guo Zhao¹ 

[1] College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China

关键词: molecular evolution;    phylogenetic analysis;    poultry;    H6 influenza viruses;   

Others  :  1155905
DOI  :  10.1186/1743-422X-8-470

 received in 2011-08-28, accepted in 2011-10-14,  发布年份 2011

【 摘 要 】

Background

Although extensive data demonstrates that the majority of H6 duck isolates belonged to a single H6N2 virus lineage with a single gene constellation in southern China from 2000 to 2005, the prevalence of H6N2 virus in poultry in Eastern China is largely unknown.

Results

Epidemiology revealed that H6N2 viruses were the most frequently detected influenza subtypes in live bird markets from 2002 to 2008 in Eastern China, but from 2009 onwards, they were replaced with novel H6N6 viruses. We phylogenetically and antigenically analyzed 42 H6 viruses isolated mainly in domestic ducks from 2002 to 2010 in Eastern China. Surprisingly, none of these isolates grouped with the previously described H6N2 viruses which belonged to a single H6N2 virus lineage with a single gene constellation in domestic ducks in southern China from 2000 to 2005. Two distinct hemagglutinin lineages were identified and they all underwent frequent reassortment with multiple virus subtypes from the natural gene pool, but few reassortants were persistent or prevalent.

Conclusions

Five subtypes of H6 influenza viruses (H6N1, H6N2, H6N5, H6N6 and H6N8) cocirculated in Eastern China, which form a significant part of the natural influenza virus reservoir in domestic ducks, and significant viral reassortment is still ongoing in this species.

【 授权许可】

   
2011 Zhao et al; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Chin PS, Hoffmann E, Webby R, Webster RG, Guan Y, Peiris M, Shortridge KF: Molecular evolution of H6 influenza viruses from poultry in Southeastern China: prevalence of H6N1 influenza viruses possessing seven A/Hong Kong/156/97 (H5N1)-like genes in poultry. J Virol 2002, 76:507-516.
• [2]Hoffmann E, Stech J, Leneva I, Krauss S, Scholtissek C, Chin PS, Peiris M, Shortridge KF, Webster RG: Characterization of the influenza A virus gene pool in avian species in southern China: was H6N1 a derivative or a precursor of H5N1? J Virol 2000, 74:6309-6315.
• [3]Lin YP, Shaw M, Gregory V, Cameron K, Lim W, Klimov A, Subbarao K, Guan Y, Krauss S, Shortridge K, Webster R, Cox N, Hay A: Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc Natl Acad Sci USA 2000, 97:9654-9658.
• [4]Peiris M, Yuen KY, Leung CW, Chan KH, Ip PL, Lai RW, Orr WK, Shortridge KF: Human infection with influenza H9N2. Lancet 1999, 354:916-917.
• [5]Scholtissek C, Burger H, Kistner O, Shortridge KF: The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses. Virology 1985, 147:287-294.
• [6]Lee MS, Chang PC, Shien JH, Cheng MC, Chen CL, Shieh HK: Genetic and pathogenic characterization of H6N1 avian influenza viruses isolated in Taiwan between 1972 and 2005. Avian Dis 2006, 50:561-571.
• [7]Huang K, Bahl J, Fan XH, Vijaykrishna D, Cheung CL, Webby RJ, Webster RG, Chen H, Smith GJ, Peiris JS, Guan Y: Establishment of an H6N2 influenza virus lineage in domestic ducks in southern China. J Virol 2010, 84:6978-6986.
• [8]Cheung CL, Vijaykrishna D, Smith GJ, Fan XH, Zhang JX, Bahl J, Duan L, Huang K, Tai H, Wang J, Poon LL, Peiris JS, Chen H, Guan Y: Establishment of influenza A virus (H6N1) in minor poultry species in southern China. J Virol 2007, 81:10402-10412.
• [9]Webby RJ, Woolcock PR, Krauss SL, Webster RG: Reassortment and interspecies transmission of North American H6N2 influenza viruses. Virology 2002, 295:44-53.
• [10]Gu M, Liu W, Cao Y, Peng D, Wang X, Wan H, Zhao G, Xu Q, Zhang W, Song Q, Li Y, Liu X: Novel Reassortant Highly Pathogenic Avian Influenza (H5N5) Viruses in Domestic Ducks, China. Emerg Infect Dis 2011, 17:1060-1063.
• [11]Stieneke-Grober A, Vey M, Angliker H, Shaw E, Thomas G, Roberts C, Klenk HD, Garten W: Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease. EMBO J 1992, 11:2407-2414.
• [12]Ha Y, Stevens DJ, Skehel JJ, Wiley DC: X-ray structures of H5 avian and H9 swine influenza virus hemagglutinins bound to avian and human receptor analogs. Proc Natl Acad Sci USA 2001, 98:11181-11186.
• [13]Scholtissek C, Quack G, Klenk HD, Webster RG: How to overcome resistance of influenza A viruses against adamantane derivatives. Antiviral Res 1998, 37:83-95.
• [14]Suzuki H, Saito R, Masuda H, Oshitani H, Sato M, Sato I: Emergence of amantadine-resistant influenza A viruses: epidemiological study. J Infect Chemother 2003, 9:195-200.
• [15]Katz JM, Lu X, Tumpey TM, Smith CB, Shaw MW, Subbarao K: Molecular correlates of influenza A H5N1 virus pathogenesis in mice. J Virol 2000, 74:10807-10810.
• [16]Massin P, van der Werf S, Naffakh N: Residue 627 of PB2 is a determinant of cold sensitivity in RNA replication of avian influenza viruses. J Virol 2001, 75:5398-5404.
• [17]Li Z, Chen H, Jiao P, Deng G, Tian G, Li Y, Hoffmann E, Webster RG, Matsuoka Y, Yu K: Molecular basis of replication of duck H5N1 influenza viruses in a mammalian mouse model. J Virol 2005, 79:12058-12064.
• [18]Qi X, Li X, Rider P, Fan W, Gu H, Xu L, Yang Y, Lu S, Wang H, Liu F: Molecular characterization of highly pathogenic H5N1 avian influenza A viruses isolated from raccoon dogs in China. PLoS One 2009, 4:e4682.
• [19]Spackman E, Stallknecht DE, Slemons RD, Winker K, Suarez DL, Scott M, Swayne DE: Phylogenetic analyses of type A influenza genes in natural reservoir species in North America reveals genetic variation. Virus Res 2005, 114:89-100.
• [20]Bahl J, Vijaykrishna D, Holmes EC, Smith GJ, Guan Y: Gene flow and competitive exclusion of avian influenza A virus in natural reservoir hosts. Virology 2009, 390:289-297.
• [21]Dugan VG, Chen R, Spiro DJ, Sengamalay N, Zaborsky J, Ghedin E, Nolting J, Swayne DE, Runstadler JA, Happ GM, Senne DA, Wang R, Slemons RD, Holmes EC, Taubenberger JK: The evolutionary genetics and emergence of avian influenza viruses in wild birds. PLoS Pathog 2008, 4:e1000076.
• [22]Lee MS, Chang PC, Shien JH, Cheng MC, Shieh HK: Identification and subtyping of avian influenza viruses by reverse transcription-PCR. J Virol Methods 2001, 97:13-22.
• [23]Qiu BF, Liu WJ, Peng DX, Hu SL, Tang YH, Liu XF: A reverse transcription-PCR for subtyping of the neuraminidase of avian influenza viruses. J Virol Methods 2009, 155:193-198.
• [24]Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR: Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 2001, 146:2275-2289.