Evolution of African cassava mosaic virus by recombination between bipartite and monopartite begomoviruses

Virology Journal

Jean-Michel Lett³  Oumar Traoré¹  Bernard Reynaud³  Nicolas Barro⁴  Alfred S Traoré⁴  Gnissa Konaté¹  Valentin SE Traoré¹  Julie Villemot³  Alexandre De Bruyn³  Mireille A Harimalala²  Murielle Hoareau³  Pierre Lefeuvre³  Fidèle Tiendrébéogo⁴ 

[1] Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476, Ouagadougou 01, Burkina Faso;Université de La Réunion, UMR PVBMT, Pôle de Protection des Plantes, Saint-Pierre, Ile de La Réunion 97410, France;CIRAD, UMR PVBMT, Pôle de Protection des Plantes, Saint-Pierre, Ile de La Réunion 97410, France;CRSBAN/UFR/SVT, Université de Ouagadougou, 03 BP 7021, Ouagadougou 03, Burkina Faso

关键词: Recombination;    Evolution;    Begomovirus;    African cassava mosaic virus;    Cassava mosaic disease;   

Others  :  1154866
DOI  :  10.1186/1743-422X-9-67

 received in 2011-12-16, accepted in 2012-03-14,  发布年份 2012

【 摘 要 】

Background

Cassava mosaic disease (CMD) is a major constraint on cassava cultivation in Africa. The disease is endemic and is caused by seven distinct cassava mosaic geminiviruses (CMGs), some of them including several variants.

Findings

From cassava leaf samples presenting CMD symptoms collected in Burkina Faso, four DNA-A begomovirus components were cloned and sequenced, showing 99.9% nucleotide identity among them. These isolates are most closely related to African cassava mosaic virus (ACMV) but share less than 89% nucleotide identity (taxonomic threshold) with any previously described begomovirus. A DNA-B genomic component, sharing 93% nucleotide identity with DNA-B of ACMV, was also characterized. Since all genomic components have a typical genome organization of Old World bipartite begomoviruses, this new species was provisionally named African cassava mosaic Burkina Faso virus (ACMBFV). Recombination analysis of the new virus demonstrated an interspecies recombinant origin, with major parents related to West African isolates of ACMV, and minor parents related to Tomato leaf curl Cameroon virus and Cotton leaf curl Gezira virus.

Conclusion

This is the first report of an ACMV-like recombinant begomovirus arisen by interspecific recombination between bipartite and monopartite African begomoviruses.

【 授权许可】

   
2012 Tiendrébéogo et al; licensee BioMed Central Ltd.

【 图 表 】

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