Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo

Virology Journal

Ruibing Cao¹  Yuexin Jin¹  Dan Zhu¹  Peng Li¹  Gang Meng¹  Weiting Liu¹  Jingman Wang¹  Hailiang Sun¹  Xue Lian¹  Yu Zhang¹  Jinyan Gu¹ 

[1] Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Tong Wei Road 6#, Nanjing 210095, Jiangsu, China

关键词: Regulation;    Replication;    IFN-α;    ISRE;    Prep;    PCV2;   

Others  :  1154221
DOI  :  10.1186/1743-422X-9-152

 received in 2011-11-06, accepted in 2012-07-27,  发布年份 2012

【 摘 要 】

Background

Porcine circovirus type 2 (PCV2) is associated with post-weaning multi-systemic wasting syndrome (PMWS) in young weaned pigs. Immune stimulation was found to activate the replication of PCV2 and exacerbate the clinical outcome of the infection. Proper amount of interferon-α (IFN-α) is able to enhance PCV2 infection and production in Porcine kidney-15 (PK-15) cells when administered after inoculation.

Methods

In the present study, luciferase reporter assays, construction of mutant viruses, Analysis the replication efficiency and the response to IFN-α treatment in PK-15 cells and animal experiments were carried out to analyze the function of interferon-stimulated response element (ISRE) of PCV2 and its role during viral replication in vitro and in vivo.

Results

A functional viral ISRE sequence, 5′-CTGAAAACGAAAGA-3′, was identified in Rep gene promoter (Prep) of PCV2. PCV2 Prep is composed of two mini promoters, the proximal one span the sequence +1 to -106, containing an ISRE while the distal mini promoter is composed of three tandem GC box like sites locate at -85 to -194. It was demonstrated that viral ISRE is necessary for porcine IFN-α initiated luciferase expression enhancement and it plays an important role in affecting the replication efficiency of PCV2 in vivo and in vitro.

Conclusions

These findings provide a theoretical basis for the Phenomenon of immunostimulation is able to enhance PCV2 infection, and improve the understanding of the complicated mechanisms involved in the host and pathogen interactions of PCV2.

【 授权许可】

   
2012 Gu et al.; licensee BioMed Central Ltd.

【 图 表 】

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