Repellent and insecticidal efficacy of a new combination of fipronil and permethrin against stable flies (Stomoxys calcitrans)

Particle and Fibre Toxicology

Mark D Soll¹  S Theodore Chester¹  Morgan L Stone¹  Jennifer P Irwin¹  Becky Fankhauser¹ 

[1] Merial Limited, 3239 Satellite Blvd, Duluth 30096, GA, USA

关键词: Insecticidal efficacy;    Repellency;    Fipronil;    Permethrin;    Dogs;    Stable flies;    Stomoxys calcitrans;   

Others  :  1147464
DOI  :  10.1186/s13071-015-0688-6

 received in 2015-01-20, accepted in 2015-01-21,  发布年份 2015

【 摘 要 】

Background

A laboratory study was conducted to assess the repellent and insecticidal efficacy of a new combination of fipronil and permethrin (Frontline Tri- Act®/Frontect® Merial) against Stomoxys calcitrans (stable flies).

Methods

Sixteen dogs were allocated to two treatment groups. Eight dogs were treated with a new topical spot-on formulation containing 6.76% w/v fipronil + 50.48% w/v permethrin on Day 0 and eight dogs served as untreated controls. Each dog was exposed to approximately 100 stable flies on Days 1, 7, 14, 21, 28 and 35. After a one-hour exposure period, live flies were carefully aspirated into a vial, anesthetized with CO₂ and crushed to determine feeding status (fed or unfed). Any dead flies remaining on the dog or in the cage were crushed to determine feeding status and counted as fed or unfed. Repellency was defined as the percent reduction in the number of fed flies in the treated group as compared to the untreated control group, and insecticidal efficacy was defined as the reduction in the number of live flies at the end of each exposure period in the treated group as compared to the control group.

Results

Percent repellency was ≥96.6% through Day 28, and 88.7% on Day 35. Percent insecticidal efficacy was ≥ 98.3% through Day 35.

Conclusions

A single topical administration of a new combination of fipronil and permethrin provides protection (repellency and insecticidal efficacy) from S. calcitrans on dogs for at least 5 weeks.

【 授权许可】

   
2015 Fankhauser et al.; licensee BioMed Central.

【 参考文献 】

• [1]Bowman DD. Arthropods. In: Georgis’ Parasitology for Veterinarians. 9th ed. Bowman DD, editor. Elsevier Saunders, St. Louis, Mo; 2009: p.5-83.
• [2]Otranto D, Cantacessi C, Testini G, Lia RP. Phortica variegata as an intermediate host of Thelazia callipaeda under natural conditions: Evidence for pathogen transmission by a male arthropod vector. Int J Parasitol. 2006; 36:1167-1173.
• [3]Yeruham I, Braverman Y. Skin lesions in dogs, horses and calves caused by the stable fly Stomoxys calcitrans (L.) (Diptera: Muscidae). Rev Elev Med Vet Pays Trop. 1995; 48:347-350.
• [4]Hogsette JA, Ruff JP, Jones CJ. Stable fly biology and control in northwest Florida. J Agric Entomol. 1987; 4:1-11.
• [5]Angarano DW. Disease of the Pinna. Vet Clin North Am Small Anim Pract. 1988; 18:869-884.
• [6]Fourie LJ, Stanneck D, Horak IG. The efficacy of a topically applied combination of imidacloprid and permethrin against Stomoxys calcitrans on dogs. Int J Appl Res Vet Med. 2006; 4:29-33.
• [7]Urban JE, Broce A. Flies and their bacterial loads in greyhound dog kennels in Kansas. Curr Microbiol. 1998; 36:164-170.
• [8]Bailie HD, Morgan DW. Field trials to assess the efficacy of permethrin for the control of flies on cattle. Vet Rec. 1980; 106:124-127.
• [9]Hogsette JA, Ruff JP. Evaluation of flucythrinate- and fenvalerate-impregnated ear tags and permethrin ear tapes for fly (Diptera: Muscidae) control on beef and dairy cattle in northwest Florida. J Econ Entomol. 1986; 79:152-157.
• [10]Hogsette JA, Ruff JP. Control of stable flies and horn flies (Diptera: Muscidae) with permethrin tapes applied to tails of beef and dairy cattle. J Econ Entomol. 1987; 80:417-420.
• [11]Beugnet F, Franc M. Results of a European multicentric field efficacy study of fipronil-(S) methoprene combination on flea infestation of dogs and cats during 2009 summer. Parasite. 2010; 17:337-342.
• [12]Brianti E, Pennisi MG, Brucato G, Risitano AL, Gaglio G, Lombardo G, Malara D, Fogliazza A, Giannetto A. Efficacy of the fipronil 10% + (S)-methoprene 9% combination against Rhipicephalus sanguineus in naturally infested dogs: Speed of kill, persistent efficacy on immature and adult stages and effect of water. Vet Parasitol. 2010; 170:96-103.
• [13]Bonneau S, Gupta S, Maynard L, Eun H-M. Antiparasitic efficacy of a new fipronil-based spot-on formulation on dogs experimentally infested with ticks Dermacentor reticulatus. Int J Appl Res Vet Med. 2011; 9:1-5.
• [14]Kužner J, Turk S, Grace S, Soni-Gupta J, Fourie JJ, Marchiondo AA, Rugg D. Confirmation of the efficacy of a novel fipronil spot-on for the treatment and control of fleas, ticks and chewing lice on dogs. Vet Parasitol. 2013; 193:245-251.
• [15]Good Clinical Practices (GCP) VICH Guideline 9. 2001.
• [16]Marchiondo AA, Holdsworth PA, Green P, Blagburn L, Jacobs DE. World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestation on dogs and cats. Vet Parasitol. 2007; 145:332-344.
• [17]Hollander M, Wolfe DA. Nonparametric Statistical Methods. John Wiley & Sons, Inc., New York; 1973.