Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia

Particle and Fibre Toxicology

Catherine Cêtre-Sossah^1,11  Renaud Lancelot^1,11  Thierry Baldet^1,11  Doreen Werner⁷  Kathrin Lehmann²  Bernd Hoffmann^1,15  Helge Kampen^1,15  Jan Schwenkenbecher⁴  Victor Sarto i Monteys^1,14  Gill De Deken^1,12  Dirk Geysen^1,12  Javier Lucientes⁵  Jorge H Calvo¹  Salah Hammami⁹  Soufien Sghaier⁹  Nonito Pagès^1,16  Federica Monaco⁸  Maria Goffredo⁸  David Borràs Borràs³  Miguel Miranda³  James Barber^1,13  Nick Golding^1,13  Laetitia Gardès^1,11  Ignace Rakotoarivony^1,11  Aurélie Pédarrieu^1,11  Rudy Meiswinkel⁶  Jean-Claude Delécolle^1,10  Simon Carpenter^1,13  Thomas Balenghien^1,11  Claire Garros^1,11 

[1] CITA-ARAID, Centro de Investigacion y Tecnologia Agroalimentaria de Aragon, Zaragoza, Spain;Humboldt-Universität zu Berlin, Cytogenetics, Chausseestr 117, Berlin, Germany;Laboratory of Zoology, UIB, University of Balearic Islands, Palma de Mallorca, Spain;IBES, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland;UZ, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain;CIDC, Central Institute for Animal diseases, Lelystad, Netherlands;Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, Germany;IZS, Istituto Zooprofilatico Sperimentale dell’Abruzzo e del Molise “G Caporale”, Teramo, Italy;IRVT, Institut de la Recherche Vétérinaire de Tunisie, Université Tunis El Manar, Tunis, Tunisia;Faculté de Médecine de Strasbourg, IPPTS, Strasbourg, France;INRA, UMR1309 CMAEE, 34398 Montpellier, France;Institute of Tropical Medicine, Antwerp, Belgium;The Pirbright Institute, Pirbright, UK;Institut de Ciència i Tecnologia Ambientals (ICTA), Bellaterra, Spain;FLI, Friedrich-Loeffler Institut, Greifswald-Insel Riems, Germany;Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain

关键词: Molecular identification;    Obsoletus group;    Subgenus Avaritia;    Culicoides;   

Others  :  806718
DOI  :  10.1186/1756-3305-7-223

 received in 2014-02-04, accepted in 2014-04-28,  发布年份 2014

【 摘 要 】

Background

Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly.

Methods

In this study, designed as an inter-laboratory ring trial with twelve partners from Europe and North Africa, we assess four PCR-based assays which are used routinely to differentiate the four species of Culicoides listed above. The assays based on mitochondrial or ribosomal DNA or microarray hybridisation were tested using aliquots of Culicoides DNA (extracted using commercial kits), crude lysates of ground specimens and whole Culicoides (265 individuals), and non-Culicoides Ceratopogonidae (13 individuals) collected from across Europe.

Results

A total of 800 molecular assays were implemented. The in-house assays functioned effectively, although specificity and sensitivity varied according to the molecular marker and DNA extraction method used. The Obsoletus group specificity was overall high (95-99%) while the sensitivity varied greatly (59.6-100%). DNA extraction methods impacted the sensitivity of the assays as well as the type of sample used as template for the DNA extraction.

Conclusions

The results are discussed in terms of current use of species diagnostic assays and the future development of molecular tools for the rapid differentiation of cryptic Culicoides species.

【 授权许可】

   
2014 Garros et al.; licensee BioMed Central Ltd.

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