Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012

Virology Journal

Changwen Ke³  Wanli Zhang³  Cong Ma³  Juan Su³  Wei Li³  Corina Monagin⁴  Pingxi Chen²  Ruiyu Chen¹  Dawei Guan³  Hong Xiao³ 

[1] Luoding Center for Disease Control and Prevention, Luoding 527221, Guangdong, China;Luoding People’s Hospital, Luoding 527221, Guangdong, China;Key Laboratory for Emergency Pathogen Detection, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong, China;Metabiota, San Francisco, California 94104, USA

关键词: Phylogenetic tree;    VP1 gene;    Aseptic meningitis;    Enterovirus;   

Others  :  826088
DOI  :  10.1186/1743-422X-10-263

 received in 2013-03-18, accepted in 2013-08-20,  发布年份 2013

【 摘 要 】

Background

Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. An outbreak of aseptic meningitis occurred from May to June, 2012, in Guangdong Province, China. In order to determine the etiologic agent, CSF specimens from 121 children hospitalized for aseptic meningitis at Luoding People’s Hospital of Guangdong Province were tested for virus isolation and identification.

Results

Enterovirus RNA was positive in 62.0% of 121 CSF sspecimens by real-time polymerase chain reaction (RT-PCR). Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of lineage groups C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups. Most of the isolates were segregated from other lineage groups. Four E30 isolates causing this outbreak aggregated into the Lineage A cluster which was derived from E30 strains that circulated in other regions of China from 2003–2010.

Conclusions

This study demonstrated the Luoding strains were a distinct lineage of E30, and a probable cause of this outbreak. The study also demonstrated that different E30 variants existed in the local meningitis outbreak.

【 授权许可】

   
2013 Xiao et al.; licensee BioMed Central Ltd.

【 图 表 】

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