Emergence of Japanese encephalitis virus genotype V in the Republic of Korea

Virology Journal

Brian P Evans⁴  John Grieco⁵  Won-Ja Lee²  Terry A Klein¹  Ampornpan Kengluecha⁴  Bousaraporn Tippayachai⁴  Heung-Chul Kim³  Ratree Takhampunya⁴ 

[1] Force Health Protection and Preventive Medicine, 65th Medical Brigade/USAMEDDAC-Korea, Unit 15281, APO AP 96205-5281, Yongsan, Seoul, Republic of Korea;National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongwon gun, Chungbuk Province, 363-951, Republic of Korea;5th Medical Detachment, 168th Multifunctional Medical Battalion, 65th Medical Brigade, Unit 15247, APO AP 96205-5247, Yongsan, Seoul, Republic of Korea;Department of Entomology, United States Army Medical Component, Armed Forces, Research Institute of Medical Sciences, APO AP 96546, Bangkok, Thailand;Department of Preventive Medicine and Biometrics, Uniformed Services University of Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA

关键词: Muar;    Culex bitaeniorhynchus;    Culex tritaeniorhynchus;    genotype V;    genotype I;    Japanese encephalitis virus;   

Others  :  1156042
DOI  :  10.1186/1743-422X-8-449

 received in 2011-08-08, accepted in 2011-09-23,  发布年份 2011

【 摘 要 】

Background

Japanese encephalitis virus (JEV) genotype V reemerged in Asia (China) in 2009 after a 57-year hiatus from the continent, thereby emphasizing a need to increase regional surveillance efforts. Genotypic characterization was performed on 19 JEV-positive mosquito pools (18 pools of Culex tritaeniorhynchus and 1 pool of Cx. bitaeniorhynchus) from a total of 64 positive pools collected from geographically different locations throughout the Republic of Korea (ROK) during 2008 and 2010.

Findings

Two regions of the JEV genome were sequenced from 19 pools; the envelope gene and the nonstructural protein 5 (NS5)/3'-untranslated region (UTR). Eighteen pools of Culex tritaeniorhynchus and one pool of Cx. bitaeniorhynchus were positive for genotype I and genotype V, respectively. Sequence alignment of the complete E gene from Cx. bitaeniorhynchus showed high amino acid similarity (98.8%) to the Muar strain, characterized as the first report of genotype V, isolated from an encephalitis patient in Malaysia in 1952.

Conclusion

This study represents the first report of JEV genotype V in the ROK. The reemergence of genotype V in Asia (China and ROK) after more than a half-century and its discovery in Cx. bitaeniorhynchus, a mosquito species previously unknown to carry JEV in the ROK, emphasizes the need for enhanced JE surveillance to monitor the dynamics of JEV strains within the region. Future findings may have implications with regard to JEV vaccination/prevention strategies.

【 授权许可】

   
2011 Takhampunya et al; licensee BioMed Central Ltd.

【 图 表 】

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