Antiviral activity of carnosic acid against respiratory syncytial virus

Virology Journal

Kyung-Soo Inn²  Dae Sik Jang²  Kyung-Tae Lee¹  Jun Chang³  Hye-Eun Choi¹  Hye-In Kim²  Na-Rae Lee²  Byeol Ryu²  Myung-Soo Choi²  Han-Bo Shin² 

[1] Department of Pharmaceutical Biochemistry and Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea;Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University, College of Pharmacy, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea;College of Pharmacy, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Korea

关键词: Anti-hRSV activity;    Carnosic acid;    Rosemarinus officinalis;    hRSV;   

Others  :  820584
DOI  :  10.1186/1743-422X-10-303

 received in 2013-06-25, accepted in 2013-10-02,  发布年份 2013

【 摘 要 】

Background

Human respiratory syncytial virus (hRSV) is a leading cause of severe lower respiratory infection and a major public health threat worldwide. To date, no vaccine or effective therapeutic agent has been developed. In a screen for potential therapeutic agents against hRSV, we discovered that an extract of Rosmarinus officinalis exerted a strong inhibitory effect against hRSV infection. Subsequent studies identified carnosic acid as a bioactive constituent responsible for anti-hRSV activity. Carnosic acid has been shown to exhibit potent antioxidant and anti-cancer activities. Anti-RSV activity of carnosic acid was further investigated in this study.

Methods

Effects of extracts from various plants and subfractions from R. officinalis on hRSV replication were determined by microneutralization assay and plaque assay. Several constituents were isolated from ethyl acetate fraction of R. officinalis and their anti-RSV activities were assessed by plaque assay as well as reverse-transcription quantitative PCR to determine the synthesis of viral RNAs.

Results

Among the tested bioactive constituents of R. officinalis, carnosic acid displayed the most potent anti-hRSV activity and was effective against both A- and B-type viruses. Carnosic acid efficiently suppressed the replication of hRSV in a concentration-dependent manner. Carnosic acid effectively suppressed viral gene expression without inducing type-I interferon production or affecting cell viability, suggesting that it may directly affect viral factors. A time course analysis showed that addition of carnosic acid 8 hours after infection still effectively blocked the expression of hRSV genes, further suggesting that carnosic acid directly inhibited the replication of hRSV.

Conclusions

The current study demonstrates that carnosic acid, a natural compound that has already been shown to be safe for human consumption, has anti-viral activity against hRSV, efficiently blocking the replication of this virus. Carnosic acid inhibited both A- and B- type hRSV, while it did not affect the replication of influenza A virus, suggesting that its antiviral activity is hRSV-specific. Collectively, this study suggests the need for further evaluation of carnosic acid as a potential treatment for hRSV.

【 授权许可】

   
2013 Shin et al.; licensee BioMed Central Ltd.

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