期刊论文详细信息
Plant Methods
Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape
Darren M Wells1  Tony P Pridmore1  Susana Ubeda-Tomás1  Ute Voß1  Daniela Dietrich1  Kim Kenobi1  Michael H Wilson1  Andrew P French1 
[1] Centre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Nottingham LE12 5RD, UK
关键词: Quantification;    Software;    Arabidopsis;    Confocal;    Image analysis;   
Others  :  822656
DOI  :  10.1186/1746-4811-8-7
 received in 2011-11-23, accepted in 2012-03-02,  发布年份 2012
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【 摘 要 】

Background

The ability to quantify the geometry of plant organs at the cellular scale can provide novel insights into their structural organization. Hitherto manual methods of measurement provide only very low throughput and subjective solutions, and often quantitative measurements are neglected in favour of a simple cell count.

Results

We present a tool to count and measure individual neighbouring cells along a defined file in confocal laser scanning microscope images. The tool allows the user to extract this generic information in a flexible and intuitive manner, and builds on the raw data to detect a significant change in cell length along the file. This facility can be used, for example, to provide an estimate of the position of transition into the elongation zone of an Arabidopsis root, traditionally a location sensitive to the subjectivity of the experimenter.

Conclusions

Cell-o-tape is shown to locate cell walls with a high degree of accuracy and estimate the location of the transition feature point in good agreement with human experts. The tool is an open source ImageJ/Fiji macro and is available online.

【 授权许可】

   
2012 French et al; licensee BioMed Central Ltd.

【 预 览 】
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