Anoctamin-1 Cl − channels in nociception: activation by an N-aroylaminothiazole and capsaicin and inhibition by T16A[inh]-A01

Molecular Pain

Bret F. Bessac¹  Farah Deba¹ 

[1] Department of Pharmaceutical Sciences, I. L. Rangel College of Pharmacy, Texas A&M Health Science Center, 1010 West Avenue B MSC 131, Kingsville 78363, TX, USA

关键词: Pain;    TRPV1;    DRG neuron;    Nociception;    Aroylaminothiazole;    T16Ainh-A01;    TMEM16A;    ANO1;    Anoctamin;   

Others  :  1228797
DOI  :  10.1186/s12990-015-0061-y

 received in 2015-03-11, accepted in 2015-09-07,  发布年份 2015

【 摘 要 】

Background

Anoctamin 1 (ANO1 or TMEM16A) Ca ²⁺ -gated Cl ⁻channels of nociceptor neurons are emerging as important molecular components of peripheral pain transduction. At physiological intracellular Cl ⁻concentrations ([Cl ⁻ ] _i ) sensory neuronal Cl ⁻channels are excitatory. The ability of sensory neuronal ANO1 to trigger action potentials and subsequent nocifensive (pain) responses were examined by direct activation with an N-aroylaminothiazole. ANO1 channels are also activated by intracellular Ca ²⁺([Ca ²⁺ ] _i ) from sensory neuronal TRPV1 (transient-receptor-potential vallinoid 1) ion channels and other noxicant receptors. Thus, sensory neuronal ANO1 can facilitate TRPV1 triggering of action potentials, resulting in enhanced nociception. This was investigated by reducing ANO1 facilitation of TRPV1 effects with: (1) T16A[inh]-A01 ANO1-inhibitor reagent at physiological [Cl ⁻ ] _iand (2) by lowering sensory neuronal [Cl ⁻ ] _ito switch ANO1 to be inhibitory.

Results

ANO1 effects on action potential firing of mouse dorsal root ganglia (DRG) neurons in vitro and mouse nocifensive behaviors in vivo were examined with an N-aroylaminothiazole ANO1-activator (E-act), a TRPV1-activator (capsaicin) and an ANO1-inhibitor (T16A[inh]-A01). At physiological [Cl ⁻ ] _i(40 mM), E-act (10 µM) increased current sizes (in voltage-clamp) and action potential firing (in current-clamp) recorded in DRG neurons using whole-cell electrophysiology. To not disrupt TRPV1 carried-Ca ²⁺activation of ANO1 in DRG neurons, ANO1 modulation of capsaicin-induced action potentials was measured by perforated-patch (Amphotericin–B) current-clamp technique. Subsequently, at physiological [Cl ⁻ ] _i , capsaicin (15 µM)-induced action potential firing was diminished by co-application with T16A[inh]-A01 (20 µM). Under conditions of low [Cl ⁻ ] _i(10 mM), ANO1 actions were reversed. Specifically, E-act did not trigger action potentials; however, capsaicin-induced action potential firing was inhibited by co-application of E-act, but was unaffected by co-application of T16A[inh]-A01. Nocifensive responses of mice hind paws were dramatically induced by subcutaneous injections of E-act (5 mM) or capsaicin (50 µM). The nocifensive responses were attenuated by co-injection with T16A[inh]-A01 (1.3 mM).

Conclusions

An ANO1-activator (E-act) induced [Cl ⁻ ] _i -dependent sensory neuronal action potentials and mouse nocifensive behaviors; thus, direct ANO1 activation can induce pain perception. ANO1-inhibition attenuated capsaicin-triggering of action potentials and capsaicin-induced nocifensive behaviors. These results indicate ANO1 channels are involved with TRPV1 actions in sensory neurons and inhibition of ANO1 could be a novel means of inducing analgesia.

【 授权许可】

   
2015 Deba and Bessac.

【 图 表 】

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