Integrating microRNA and mRNA expression profiles in response to radiation-induced injury in rat lung

Radiation Oncology

Jinchang Wu²  XingJun Meng²  ChuanJun Song²  Weiqun Ding¹  Rensheng Lai³  Qing Chen²  Shuyu Zhang⁴  Jundong Zhou²  Ling Xie³ 

[1] Department of Pathology, University of Oklahoma Health Science Center, Oklahoma City, OKC, USA;Department of Radio-oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou 215001, China;Department of Pathology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China

关键词: Microarray;    mRNA;    MicroRNAs;    Rat lung;    Radiation;   

Others  :  805217
DOI  :  10.1186/1748-717X-9-111

 received in 2013-12-03, accepted in 2014-04-25,  发布年份 2014

【 摘 要 】

Purpose

Exposure to radiation provokes cellular responses, which are likely regulated by gene expression networks. MicroRNAs are small non-coding RNAs, which regulate gene expression by promoting mRNA degradation or inhibiting protein translation. The expression patterns of both mRNA and miRNA during the radiation-induced lung injury (RILI) remain less characterized and the role of miRNAs in the regulation of this process has not been studied. The present study sought to evaluate miRNA and mRNA expression profiles in the rat lung after irradiation.

Methods and materials

Male Wistar rats were subjected to single dose irradiation with 20 Gy using 6 MV x-rays to the right lung. (A dose rate of 5 Gy/min was applied). Rats were sacrificed at 3, 12 and 26 weeks after irradiation, and morphological changes in the lung were examined by haematoxylin and eosin. The miRNA and mRNA expression profiles were evaluated by microarrays and followed by quantitative RT-PCR analysis.

Results

A cDNA microarray analysis found 2183 transcripts being up-regulated and 2917 transcripts down-regulated (P ≤ 0.05, ≥2.0 fold change) in the lung tissues after irradiation. Likewise, a miRNAs microarray analysis indicated 15 miRNA species being up-regulated and 8 down-regulated (P ≤ 0.05). Subsequent bioinformatics anal -yses of the differentially expressed mRNA and miRNAs revealed that alterations in mRNA expression following irradiation were negatively correlated with miRNAs expression.

Conclusions

Our results provide evidence indicating that irradiation induces alterations of mRNA and miRNA expression in rat lung and that there is a negative correlation of mRNA and miRNA expression levels after irradiation. These findings significantly advance our understanding of the regulatory mechanisms underlying the pathophysiology of radiation-induced lung injury. In summary, RILI does not develop gradually in a linear process. In fact, different cell types interact via cytokines in a very complex network. Furthermore, this study suggests that microRNAs may serve an important role in the pathogenesis of RILI and that understanding their role in RILI may have a significant effect on patient management and diagnosis in the future.

【 授权许可】

   
2014 Xie et al.; licensee BioMed Central Ltd.

【 图 表 】

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