Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophy

Nutrition & Metabolism

Jacob M Wilson²  Troy A Hornberger¹  Stephanie MC Wilson³  Michael D Roberts⁵  Martin Purpura⁴  Sean A McCleary²  Ralf Jäger⁴  Ryan P Lowery²  David M Gundermann¹  Jordan M Joy² 

[1] Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, USA;Department of Health Sciences and Human Performance, The University of Tampa, 401 W. Kennedy Blvd., Box 30 F, Tampa, FL 33606, USA;Department of Nutrition, IMG Academy, Bradenton, FL, USA;Increnovo LLC, 2138 E Lafayette Pl, Milwaukee, WI 53202, USA;School of Kinesiology, Auburn University, Auburn, AL, USA

关键词: Ergogenic aid;    Phospholipid;    Protein synthesis;    Skeletal muscle;    Supplementation;   

Others  :  801097
DOI  :  10.1186/1743-7075-11-29

 received in 2014-04-16, accepted in 2014-06-07,  发布年份 2014

【 摘 要 】

Introduction

The lipid messenger phosphatidic acid (PA) plays a critical role in the stimulation of mTOR signaling. However, the mechanism by which PA stimulates mTOR is currently unknown. Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance.

Methods

In phase one, C₂C₁₂ myoblasts cells were stimulated with different phospholipids and phospholipid precursors derived from soy and egg sources. The ratio of phosphorylated p70 (P-p70-389) to total p70 was then used as readout for mTOR signaling. In phase two, resistance trained subjects (n = 28, 21 ± 3 years, 77 ± 4 kg, 176 ± 9 cm) consumed either 750 mg PA daily or placebo and each took part in an 8 week periodized resistance training program.

Results

In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%). In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo.

Conclusion

PA significantly activates mTOR and significantly improved responses in skeletal muscle hypertrophy, lean body mass, and maximal strength to resistance exercise.

【 授权许可】

   
2014 Joy et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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