Trisomy 8: a common finding in mouse embryonic stem (ES) cell lines

Molecular Cytogenetics

Shibo Li³  John J Mulvihill³  Lijun Xia²  Ji-Yun Lee¹  Young Mi Kim³ 

[1] Department of Pathology, Korea University, Seoul, 136-705, South Korea;Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA;Department of Pediatrics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA

关键词: Mosaicism;    FISH;    Chromosomal aberrations;    Mouse ES cells;   

Others  :  1151180
DOI  :  10.1186/1755-8166-6-3

 received in 2012-08-28, accepted in 2012-12-06,  发布年份 2013

【 摘 要 】

Background

Obtaining a germ cell line is one of the most important steps in developing a transgenic or knockout mouse with a targeted mutated gene of interest. A common problem with this technology is that embryonic stem (ES) cells often lack, or are extremely inefficient at, germ line transmission.

Results

To determine whether chromosomal anomalies are correlated with inefficient ES cell germ line transmission, we examined 97 constructed ES cell lines using conventional cytogenetic analysis, and fluorescence in situ hybridization (FISH). Chromosomal abnormalities occurred in 44 (45%) out of the 97 specimens analyzed: 31 specimens had trisomy 8 or mosaic trisomy 8, eight specimens had partial trisomy 8 resulting from unbalanced translocations, and five specimens had other chromosomal anomalies.

Conclusions

Our data suggest that chromosomal analysis is an important tool for improving the yield and quality of gene targeting experiments.

【 授权许可】

   
2013 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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