Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes

Particle and Fibre Toxicology

Catherine Walton⁴  S. H. P. Parakrama Karunaratne⁵  Lalanthika B. S. Peiris⁶  Kanapathy Gajapathy¹  Ranjan Ramasamy²  Pavilupillai J. Jude¹  Devojit K. Sarma³  Nathan Truelove⁴  Sinnathamby N. Surendran⁴ 

[1] Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka;Faculty of Science and Technology, Anglia Ruskin University, Cambridge CB1 1PT, UK;Regional Medical Research Centre, NE region (ICMR), Dibrugarh 786001, Assam, India;Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK;Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya 20400, Sri Lanka;Regional Office, Anti Malaria Campaign, Hambantota 82000, Sri Lanka

关键词: Y-chromosome karyotype;    Sri Lanka;    Species complex;    Mosquito vector;    Microsatellite;    Malaria;    COI;    Anopheles culicifacies;   

Others  :  1224194
DOI  :  10.1186/s13071-015-0944-9

 received in 2015-04-22, accepted in 2015-06-08,  发布年份 2015

【 摘 要 】

Background

The identification of species B and E in the Anopheles culicifacies complex in the Indian subcontinent has been based on Y-chromosome karyotype. Since no detectable variations were previously found in DNA markers commonly used for sibling species identification, further molecular characterization using cytochrome oxidase subunit I (COI) and microsatellite markers was carried out on Y-chromosome karyotyped Anopheles culicifacies specie B and E from Unnichchai, Kallady and Ranawarunawa in Sri Lanka.

Findings

COI sequence analysis (n = 22) revealed the presence of nine unique haplotypes with six in each species. Three haplotypes were shared by both species. The two sibling species had a pairwise F _STvalue of 1.338 (p < 0.05) with the number of migrants (Nm) value <1. The genetic structure analysis resulted in two genetic clusters not 100 % associated with karyotypes. While none of the species B were incorrectly assigned two were inconclusive. Five out of 26 specimens karyotyped as species E were incorrectly assigned, while further 9 were inconclusive.

Conclusions

The new molecular data support the existence of two genetically different populations of the Culicifacies Complex in Sri Lanka that are not associated with the Y-chromosome karyotype. Detailed analysis with more microsatellite markers and assortative mating experiments are needed to establish the presence of the two genetically distinct populations and relate them to Y-chromosome morphology.

【 授权许可】

   
2015 Surendran et al.

【 图 表 】

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