Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis

Molecular Cytogenetics

Lisa G Shaffer⁴  Blake C Ballif⁴  Trilochan Sahoo⁵  S Annie Morton⁴  Steve Byerly⁴  Nicholas J Neill⁴  Sara Minier⁴  Caitlin Valentin⁴  Victoria Cawich⁴  Karl S Theil⁶  James R Cook⁶  Raymond R Tubbs⁶  Theresa C Brown¹  Lisa D McDaniel⁴  Marilyn L Slovak²  Roger A Schultz⁴  Kathryn A Kolquist³ 

[1] CSI Laboratories, 11525 Park Woods Circle, Alpharetta, GA, 30005, USA;Quest Diagnostics Nichols Institute, 14225 Newbrook Drive, Chantilly, VA, 20151, USA;Sacred Heart Medical Center, 101 West 8th Avenue, Spokane, WA, 99204, USA;Signature Genomic Laboratories, PerkinElmer Inc., 2820 North Astor Street, Spokane, WA, 99207, USA;Quest Diagnostics Nichols Institute, 33608 Ortega Highway, San Juan Capistrano, CA, 92675, USA;Cleveland Clinic, Pathology & Laboratory Medicine Institute, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

关键词: chromosome aberration;    cytogenetics;    FISH;    oligonucleotide;    microarray;    chronic lymphocytic leukemia;   

Others  :  1151941
DOI  :  10.1186/1755-8166-4-25

 received in 2011-08-26, accepted in 2011-11-16,  发布年份 2011

【 摘 要 】

Background

Cytogenetic evaluation is a key component of the diagnosis and prognosis of chronic lymphocytic leukemia (CLL). We performed oligonucleotide-based comparative genomic hybridization microarray analysis on 34 samples with CLL and known abnormal karyotypes previously determined by cytogenetics and/or fluorescence in situ hybridization (FISH).

Results

Using a custom designed microarray that targets >1800 genes involved in hematologic disease and other malignancies, we identified additional cryptic aberrations and novel findings in 59% of cases. These included gains and losses of genes associated with cell cycle regulation, apoptosis and susceptibility loci on 3p21.31, 5q35.2q35.3, 10q23.31q23.33, 11q22.3, and 22q11.23.

Conclusions

Our results show that microarray analysis will detect known aberrations, including microscopic and cryptic alterations. In addition, novel genomic changes will be uncovered that may become important prognostic predictors or treatment targets for CLL in the future.

【 授权许可】

   
2011 Kolquist et al; licensee BioMed Central Ltd.

【 图 表 】

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