Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease

World Allergy Organization Journal

Kazuhisa Ono¹  Takaharu Hayashi⁴  Tsunehiro Aki¹  Kunihiko Hashimoto²  Nobukazu Onishi²  Satoshi Rikimaru²  Yayoi Shimada³  Keisuke Mizuno¹  Seiji Kawamoto¹  Ahmed Ragaa Nour Ibrahim¹ 

[1] Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan;Nishikawa Rubber Co. Ltd., Hiroshima, Japan;JST Innovation Plaza Hiroshima, Higashi-Hiroshima, Japan;Takanobashi Central Hospital, Hiroshima, Japan

关键词: subtilisin;    serine protease;    Japanese cedar pollen;    allergen;   

Others  :  1145644
DOI  :  10.1097/WOX.0b013e318201d81d

【 摘 要 】

Protease activities in allergen sources are thought to be involved in triggering allergic inflammation through the disruption of epithelial barrier or the induction of proinflammatory cytokines. Protease allergens may also work as type 2 helper T cell (T_H2) adjuvants through the cleavage of cell surface receptors. Here, we report molecular cloning and immunochemical characterization of a new Japanese cedar (Cryptomeria japonica) pollen allergen (CPA9) homologous to serine protease, which is initially found as a high IgE-binding spot on our two-dimensional (2-D) IgE immunoblotting map. The cpa9 cDNA encoded a 757 amino acid polypeptide showing a significant sequence identity with plant subtilisin-like serine protease family members including melon major allergen Cuc m 1. We found that native CPA9 purified from C. japonica pollen showed a high IgE-binding frequency and IgE cross-reactivity with melon extract.

【 授权许可】

   
2010 World Allergy Organization; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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