Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1454

Article

Kawamoto, S ; Mizuguchi, Y ; Morimoto, K ; Aki, T ; Shigeta, S ; Yasueda, H ; Wada, T ; Suzuki, O ; Jyo, T ; Ono, K

关键词: Der f 6;    allergen;    house dust mite;    chymotrypsin;    serine protease;   

DOI  :  10.1016/S0925-4439(99)00041-1

来源: Elsevier

【 摘 要 】

House dust mite allergen is thought to be a major cause of asthma. Characterization of these allergen molecules is therefore an important step for the development of effective diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report molecular cloning and expression of the group 6 (chymotrypsin-like) allergen from the house dust mite, Dermatophagoides farinae. Sequencing analysis indicates that cloned cDNA, designated Der f 6, encodes a 279 amino acid polypeptide which conserves a primary structure characteristic for chymotrypsin-like serine proteases found in mammals. Recombinant Der f 6 expressed in Escherichia coli bound IgE in a pool made of 20 sera, and induced histamine release from patients' peripheral blood cells. (C) 1999 Elsevier Science B.V. All rights reserved.

【 授权许可】

Free