A cost effective real-time PCR for the detection of adenovirus from viral swabs

Virology Journal

Jason J LeBlanc¹  Todd F Hatchette¹  Raymond Tellier²  Kanti Pabbaraju³  Sallene Wong³  Melanie Wilcox¹  Khalifa Binkhamis⁴  Turkiya Al-Siyabi⁴ 

[1] Dalhousie University, Halifax, Nova Scotia, Canada;University of Calgary, Calgary, Alberta, Canada;Provincial Laboratory for Public Health, Calgary, Alberta, Canada;Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority Room 404B Mackenzie Building, 5788 University Ave., Halifax, NS B3H 1V8

关键词: Cost analysis;    Adenovirus;    Real-time PCR;    Extraction;    Homogenization;   

Others  :  1149882
DOI  :  10.1186/1743-422X-10-184

 received in 2013-02-27, accepted in 2013-05-23,  发布年份 2013

【 摘 要 】

Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an “in-house” real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.

【 授权许可】

   
2013 Al-Siyabi et al.; licensee BioMed Central Ltd.

【 图 表 】

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