Immunohistochemial study on the expression of von Willebrand factor (vWF) after onlay autogenous iliac grafts for lateral alveolar ridge augmentation

Head & Face Medicine

Tobias Reuther¹  Alexander C Kuebler¹  Oliver Ristow³  Wilhelm Bloch²  Joerg Siebers¹  Steffen Koerdt¹ 

[1] Department of Oral and Maxillofacial Plastic Surgery, University of Wuerzburg, Pleicherwall 2, Wuerzburg D-97070, Germany;Department of Molecular and Cellular Sport Medicine, German Sport University, Am Sportpark Muengersdorf 6, Cologne D-50933, Germany;Medicine & Aesthetics, Clinic for Oral, Maxillofacial and Plastical Surgery, Lenbachplatz 2a, Munich D-80333, Germany

关键词: vWF;    Bone graft;    Connective tissue;    Revascularization;    Collagen membrane;    GBR;   

Others  :  813592
DOI  :  10.1186/1746-160X-9-40

 received in 2013-08-05, accepted in 2013-12-03,  发布年份 2013

【 摘 要 】

Introduction

The main problems of autogenous bone transplants are their unpredictable atrophy and their loss of structure. One key factor lies in the poor revascularization of simple onlay grafts. The the aim of this study was to evaluate the revascularization processes in autogenous bone grafts from the iliac crest to the alveolar ridge.

Methods

In a sheep model, autogenous bone grafts were harvested from the iliac crest. A combination of a resorbable collagen membrane (CM) and deproteinized bovine bone material (DBBM) was used to modify the bone graft (experiment 2). This was compared with a simple onlay bone graft (control group, experiment 1). The amount of vessels in bone and connective tissue (CT), and the amount of CT were analyzed. The expression of von Willebrand factor (vWF) was compared between the two experimental groups using immunohistochemical analysis.

Results

The ratio of the amount of vessels in bone and CT changed over time, and more vessels could be detected in bone at 12–16 weeks of graft healing. The number of vessels were significantly higher in experiment 2 than in experiment 1. More CT was found in experiment 1, whereas the amount of CT in both experiments decreased over time.

Conclusion

This study shows a more intensive and extensive revascularization in experiment 2, as significantly more vessels were detected. The decreased amount of CT in experiment 2 clarifies its clinical superiority.

【 授权许可】

   
2013 Koerdt et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Bauer TW, Muschler GF: Bone graft materials. An overview of the basic science. Clin Orthop Relat Res 2000, (371):10-27.
• [2]Khan SN, Cammisa FP Jr, Sandhu HS, Diwan AD, Girardi FP, Lane JM: The biology of bone grafting. J Am Acad Orthop Surg 2005, 13:77-86.
• [3]Adeyemo WL, Reuther T, Bloch W, Korkmaz Y, Fischer JH, Zoller JE, Kuebler AC: Influence of host periosteum and recipient bed perforation on the healing of onlay mandibular bone graft: an experimental pilot study in the sheep. Oral Maxillofac Surg 2008, 12:19-28.
• [4]Adeyemo WL, Reuther T, Bloch W, Korkmaz Y, Fischer JH, Zoller JE, Kuebler AC: Healing of onlay mandibular bone grafts covered with collagen membrane or bovine bone substitutes: a microscopical and immunohistochemical study in the sheep. Int J Oral Maxillofac Surg 2008, 37:651-659.
• [5]Maiorana C, Beretta M, Salina S, Santoro F: Reduction of autogenous bone graft resorption by means of bio-oss coverage: a prospective study. Int J Periodontics Restorative Dent 2005, 25:19-25.
• [6]Phillips JH, Rahn BA: Fixation effects on membranous and endochondral onlay bone-graft resorption. Plast Reconstr Surg 1988, 82:872-877.
• [7]Alberius P, Dahlin C, Linde A: Role of osteopromotion in experimental bone grafting to the skull: a study in adult rats using a membrane technique. J Oral Maxillofac Surg 1992, 50:829-834.
• [8]Araujo MG, Sonohara M, Hayacibara R, Cardaropoli G, Lindhe J: Lateral ridge augmentation by the use of grafts comprised of autologous bone or a biomaterial. An experiment in the dog. J Clin Periodontol 2002, 29:1122-1131.
• [9]Dongieux JW, Block MS, Morris G, Gardiner D, Dean K: The effect of different membranes on onlay bone graft success in the dog mandible. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998, 86:145-151.
• [10]Donos N, Kostopoulos L, Karring T: Augmentation of the rat jaw with autogeneic cortico-cancellous bone grafts and guided tissue regeneration. Clin Oral Implants Res 2002, 13:192-202.
• [11]Donos N, Kostopoulos L, Karring T: Alveolar ridge augmentation using a resorbable copolymer membrane and autogenous bone grafts. An experimental study in the rat. Clin Oral Implants Res 2002, 13:203-213.
• [12]Donos N, Kostopoulos L, Karring T: Augmentation of the mandible with GTR and onlay cortical bone grafting. An experimental study in the rat. Clin Oral Implants Res 2002, 13:175-184.
• [13]Sahni M, Guenther HL, Fleisch H, Collin P, Martin TJ: Bisphosphonates act on rat bone resorption through the mediation of osteoblasts. J Clin Invest 1993, 91:2004-2011.
• [14]Schlegel KA, Fichtner G, Schultze-Mosgau S, Wiltfang J: Histologic findings in sinus augmentation with autogenous bone chips versus a bovine bone substitute. Int J Oral Maxillofac Implants 2003, 18:53-58.
• [15]Karring T, Nyman S, Gottlow J, Laurell L: Development of the biological concept of guided tissue regeneration--animal and human studies. Periodontol 1993, 1:26-35.
• [16]Dahlin C, Alberius P, Linde A: Osteopromotion for cranioplasty. An experimental study in rats using a membrane technique. J Neurosurg 1991, 74:487-491.
• [17]Jovanovic SA, Spiekermann H, Richter EJ: Bone regeneration around titanium dental implants in dehisced defect sites: a clinical study. Int J Oral Maxillofac Implants 1992, 7:233-245.
• [18]Kostopoulos L, Karring T: Guided bone regeneration in mandibular defects in rats using a bioresorbable polymer. Clin Oral Implants Res 1994, 5:66-74.
• [19]Lundgren D, Lundgren AK, Sennerby L, Nyman S: Augmentation of intramembraneous bone beyond the skeletal envelope using an occlusive titanium barrier. An experimental study in the rabbit. Clin Oral Implants Res 1995, 6:67-72.
• [20]Simion M, Trisi P, Piattelli A: Vertical ridge augmentation using a membrane technique associated with osseointegrated implants. Int J Periodontics Restorative Dent 1994, 14:496-511.
• [21]Koerdt S, Ristow O, Wannhoff A, Kubler AC, Reuther T: Expression of growth factors during the healing process of alveolar ridge augmentation procedures using autogenous bone grafts in combination with GTR and an anorganic bovine bone substitute: An immunohistochemical study in the sheep. Clin oral investig 2013. [Epub ahead of print]
• [22]Guillemin G, Patat JL, Fournie J, Chetail M: The use of coral as a bone graft substitute. J Biomed Mater Res 1987, 21:557-567.
• [23]Holmes RE: Bone regeneration within a coralline hydroxyapatite implant. Plast Reconstr Surg 1979, 63:626-633.
• [24]Hammerle CH, Schmid J, Lang NP, Olah AJ: Temporal dynamics of healing in rabbit cranial defects using guided bone regeneration. J Oral Maxillofac Surg 1995, 53:167-174.
• [25]Schmid J, Wallkamm B, Hammerle CH, Gogolewski S, Lang NP: The significance of angiogenesis in guided bone regeneration. A case report of a rabbit experiment. Clin Oral Implants Res 1997, 8:244-248.
• [26]Winet H: The role of microvasculature in normal and perturbed bone healing as revealed by intravital microscopy. Bone 1996, 19:39S-57S.
• [27]Glowacki J: Angiogenesis in fracture repair. Clin Orthop Relat Res 1998, (355 Suppl):S82-89.
• [28]Remmele W, Schicketanz KH: Immunohistochemical determination of estrogen and progesterone receptor content in human breast cancer. Computer-assisted image analysis (QIC score) vs. subjective grading (IRS). Pathol res pract 1993, 189:862-866.
• [29]Jardini MA, De Marco AC, Lima LA: Early healing pattern of autogenous bone grafts with and without e-PTFE membranes: a histomorphometric study in rats. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005, 100:666-673.
• [30]De Marco AC, Jardini MA, Lima LP: Revascularization of autogenous block grafts with or without an e-PTFE membrane. Int J Oral Maxillofac Implants 2005, 20:867-874.
• [31]Fonseca RJ, Clark PJ, Burkes EJ Jr, Baker RD: Revascularization and healing of onlay particulate autologous bone grafts in primates. J Oral Surg 1980, 38:572-577.
• [32]Verhoeven JW, Ruijter J, Cune MS, Terlou M, Zoon M: Onlay grafts in combination with endosseous implants in severe mandibular atrophy: one year results of a prospective, quantitative radiological study. Clin Oral Implants Res 2000, 11:583-594.
• [33]Enneking WF, Burchardt H, Puhl JJ, Piotrowski G: Physical and biological aspects of repair in dog cortical-bone transplants. J Bone Joint Surg Am 1975, 57:237-252.
• [34]Jensen OT, Greer RO Jr, Johnson L, Kassebaum D: Vertical guided bone-graft augmentation in a new canine mandibular model. Int J Oral Maxillofac Implants 1995, 10:335-344.
• [35]Lundgren AK, Lundgren D, Sennerby L, Taylor A, Gottlow J, Nyman S: Augmentation of skull bone using a bioresorbable barrier supported by autologous bone grafts. An intra-individual study in the rabbit. Clin Oral Implants Res 1997, 8:90-95.
• [36]Thorwarth M, Schlegel KA, Wehrhan F, Srour S, Schultze-Mosgau S: Acceleration of de novo bone formation following application of autogenous bone to particulated anorganic bovine material in vivo. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006, 101:309-316.
• [37]Galindo Moreno P, Moreno Riestra I, Avila G, Padial Molina M, Paya JA, Wang HL, O'Valle F: Effect of anorganic bovine bone to autogenous cortical bone ratio upon bone remodeling patterns following maxillary sinus augmentation. Clin Oral Implants Res 22(8):857-864. 10.1111/j.1600-0501.2010.02073.x. Epub 2011 Jan 18
• [38]Alles JU, Bosslet K: Immunocytochemistry of angiosarcomas. A study of 19 cases with special emphasis on the applicability of endothelial cell specific markers to routinely prepared tissues. Am j clin pathol 1988, 89:463-471.
• [39]Pusztaszeri MP, Seelentag W, Bosman FT: Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. j histochem cytochem off j Histochem Soc 2006, 54:385-395.
• [40]Wanschitz F, Nell A, Patruta S, Wagner A, Ewers R: Influence of three currently used bone replacing materials on the in vitro proliferation of human peripheral blood mononuclear cells. Clin Oral Implants Res 2005, 16:570-574.
• [41]Schwartz Z, Weesner T, van Dijk S, Cochran DL, Mellonig JT, Lohmann CH, Carnes DL, Goldstein M, Dean DD, Boyan BD: Ability of deproteinized cancellous bovine bone to induce new bone formation. J Periodontol 2000, 71:1258-1269.
• [42]Taylor JC, Cuff SE, Leger JP, Morra A, Anderson GI: In vitro osteoclast resorption of bone substitute biomaterials used for implant site augmentation: a pilot study. Int J Oral Maxillofac Implants 2002, 17:321-330.
• [43]Degidi M, Artese L, Rubini C, Perrotti V, Iezzi G, Piattelli A: Microvessel density and vascular endothelial growth factor expression in sinus augmentation using Bio-Oss. Oral dis 2006, 12:469-475.