Reconstructing a comprehensive transcriptome assembly of a white-pupal translocated strain of the pest fruit fly Bactrocera cucurbitae

GigaScience

Scott M Geib¹  Theodore DeRego¹  Brian Hall²  Bernarda Calla¹  Sheina B Sim² 

[1] Tropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI, USA;Department of Plant and Environmental Protection Sciences, University of Hawaii, Manoa, Honolulu, HI, USA

关键词: Sterile insect technique;    SIT;    Melon fly;    Tephritidae;    White-pupae;    RNA-Seq;    Translocation;    Bactrocera cucurbitae;   

Others  :  1149285
DOI  :  10.1186/s13742-015-0053-x

 received in 2014-12-31, accepted in 2015-03-09,  发布年份 2015

【 摘 要 】

Background

Bactrocera cucurbitae is a serious global agricultural pest. Basic genomic information is lacking for this species, and this would be useful to inform methods of control, damage mitigation, and eradication efforts. Here, we have sequenced, assembled, and annotated a comprehensive transcriptome for a mass-rearing sexing strain of this species. This forms a foundational genomic and transcriptomic resource that can be used to better understand the physiology and biochemistry of this insect as well as being a useful tool for population genetics.

Findings

A transcriptome assembly was constructed containing 17,654 transcript isoforms derived from 10,425 unigenes. This transcriptome size is similar to reports from other Tephritid species and probably includes about 70-80% of the protein-coding genes in the genome. The dataset is publicly available in NCBI and GigaDB as a resource for researchers.

Conclusions

Foundational knowledge on the protein-coding genes in B. cucurbitae will lead to improved resources for this species. Through comparison with a model system such as Drosophila as well as a growing number of related Tephritid transcriptomes, improved strategies can be developed to control this pest.

【 授权许可】

   
2015 Sim et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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