Butter naturally enriched in cis-9, trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats

Lipids in Health and Disease

Raúl Marcel González Garcia⁴  Fernando César Ferraz Lopes⁵  Marco Antônio Sundfeld da Gama⁵  Jacy Gameiro¹  Egberto Gaspar de Moura³  Patrícia Cristina Lisboa³  Ellen Paula Santos da Conceição³  Larissa Gomes dos Reis⁶  José Otávio do Amaral Corrêa⁶  Céphora Maria Sabarense²  Sheila Cristina Potente Dutra Luquetti²  Mariana Macedo de Almeida⁴ 

[1] Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil;Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil;Department of Physiological Sciences, Roberto Alcantara Gomes Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil;Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil;Embrapa Dairy Cattle, Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil;Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

关键词: Diabetes;    Dyslipidemia;    Insulin sensitivity;    Rats;    Functional food;    High conjugated linoleic acid enriched butter;   

Others  :  1135957
DOI  :  10.1186/1476-511X-13-200

 received in 2014-07-21, accepted in 2014-12-04,  发布年份 2014

【 摘 要 】

Background

Evidence from in vitro and animal studies indicates that conjugated linoleic acid (CLA) possesses anti-diabetic properties, which appear to be attributed to cis-9, trans-11 CLA, the major CLA isomer in ruminant fat. However, there is a shortage of studies addressing CLA from natural source. The present study aimed to evaluate the effects of butter naturally enriched in cis-9, trans-11 CLA on parameters related to glucose tolerance, insulin sensitivity and dyslipidemia in rats.

Methods

Forty male Wistar rats were randomly assigned to the following dietary treatments (n = 10/group), for 60 days: 1) Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); 2) High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; 3) High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; and 4) High fat-Soybean oil (HF-So): diet containing 24.0% SO. HF-Cb and HF-CLAb diets contained 0.075% and 0.235% of cis-9, trans-11 CLA, respectively.

Results

HF-CLAb-fed rats had lower serum insulin levels at fasting than those fed with the HF-Cb diet, while the PPARγ protein levels in adipose tissue was increased in HF-CLAb-fed rats compared to HF-Cb-fed rats. Furthermore, R-QUICK was lower in HF-Cb than in NF-So group, while no differences in R-QUICK were observed among NF-So, HF-CLAb and HF-So groups. Serum HDL cholesterol levels were higher in HF-CLAb-fed rats than in those fed NF-So, HF-Cb and HF-So diets, as well as higher in NF-So-fed rats than in HF-Cb and HF-So-fed rats. HF-CLAb, HF-Cb and HF-So diets reduced serum LDL cholesterol levels when compared to NF-So, whereas serum triacylglycerol levels were increased in HF-CLAb.

Conclusion

Feeding rats on a high-fat diet containing butter naturally enriched in cis-9, trans-11 CLA prevented hyperinsulinemia and increased HDL cholesterol, which could be associated with higher levels of cis-9, trans-11 CLA, vaccenic acid, oleic acid and lower levels of short and medium-chain saturated fatty acids from butter naturally modified compared to control butter. On the other hand CLA-enriched butter also increased serum triacylglycerol levels, which could be associated with concomitant increases in the content of trans-9 and trans-10 C18:1 isomers in the CLA-enriched butter.

【 授权许可】

   
2015 de Almeida et al.; licensee BioMed Central.

【 图 表 】

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