Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART

European Journal of Medical Research

N Wu¹  NH Brockmeyer³  A Skaletz-Rorowski³  A Potthoff³  S Hoextermann³  C Jin¹  L Feng²  Y Jin¹  N Weng¹  C Shou¹ 

[1] State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou, China;Department of Dermatology, Venerology and Allergology, St. Josef Hospital, Ruhr-University Bochum, Bochum, Germany

关键词: IL-7;    T cell subsets;    highly active antiretroviral therapy (HAART);    HIV-1;   

Others  :  824659
DOI  :  10.1186/2047-783X-16-11-473

 received in 2011-12-21, accepted in 2011-05-23,  发布年份 2011

【 摘 要 】

Objective

To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods

Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4⁺, CD8⁺, CD8/CD38⁺, and CD8/HLADR⁺ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3⁺ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results

At baseline CD4⁺ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8⁺, CD8/HLADR⁺ and CD8/CD38⁺ T cell counts were higher than those in the control group (p <0.01). After seven years of effective HAART, the CD4⁺ T cell counts had increased and the CD8⁺ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR⁺ and CD8/CD38⁺ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38⁺ T cell count had not decreased significantly, and CD8/HLADR⁺ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3⁺ CD8⁺ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3⁺ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion

After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

【 授权许可】

   
2011 I. Holzapfel Publishers

【 图 表 】

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