Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA)

BMC Veterinary Research

Marco Alberto Medeiros¹  Walter Lilenbaum³  Albert Icksang Ko²  Carolina Lessa-Aquino¹  Sabrina Thomé³ 

[1] Fiocruz, Bio-Manguinhos, Laboratory of Recombinant Technology, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, 21045-900, RJ, Brasil;School of Public Health, Department of Epidemiology of Microbial Diseases, Yale University, 60 College Street, Downtown, New Haven, 06510, USA;Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil

关键词: Lig proteins;    LipL32;    MAPIA;    Dogs;    Leptospirosis;   

Others  :  1092028
DOI  :  10.1186/s12917-014-0288-2

 received in 2014-07-12, accepted in 2014-11-24,  发布年份 2014

【 摘 要 】

Background

The microscopic agglutination test (MAT), the standard method for serological diagnosis of leptospirosis, may present limitations regarding its sensitivity. Current studies suggest that Leptospira immunoglobulin-like (Lig) proteins and LipL32 are of particular interest as serodiagnostic markers since they are present only in pathogenic species of the Leptospira genus. The purpose of this study was to identify leptospiral immunodominant proteins that are recognized by canine sera from diseased dogs.

Results

A total of 109 dogs were studied, including seroreactive dogs (MAT ≥800) and dogs with no seroreactivity detectable by MAT. Eight recombinant fragments (31–70 kDa) of pathogenic Leptospira were tested for their use as diagnostic markers for canine leptospirosis using the Multi-antigen Print Immunoassay (MAPIA) platform: LigB [582-947aa] from L. interrogans serovar Pomona, L. interrogans serovar Copenhageni and L. kirschneri serovar Gryppotyphosa, LigB [131-649aa] from L. interrogans serovar Copenhageni, L. interrogans serovar Canicola and L. kirschneri serovar Gryppotyphosa, LigA [625-1224aa] L. interrogans serovar Copenhageni and LipL32 from L. interrogans serovar Copenhageni. The data were analyzed and ROC curves were generated. Altogether, LigB [131-649aa] L. interrogans Canicola, LigB [131-649aa] L. kirschneri Gryppotyphosa and LipL32 L. interrogans Copenhageni showed best accuracy (AUC = 0.826 to 0.869), with 70% specificity and sensitivity ranging from 89% to 95%.

Conclusions

These results reinforce their potential as diagnostic candidates for the development of new methods for the serological diagnosis of canine leptospirosis.

【 授权许可】

   
2014 Thomé et al.; licensee BioMed Central Ltd.

【 图 表 】

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