Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis

Particle and Fibre Toxicology

Patrícia Sampaio Tavares Veras⁴  Deborah Bittencourt Mothé Fraga³  Gabriel Grimaldi²  Alexandre Barbosa Reis¹  Edmilson Domingos da Silva⁵  Michel Vergne Sucupira⁵  Rodrigo Araujo Silva²  Isaac Queiroz de Oliveira² 

[1] Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil;Laboratório de Patologia e Biointervenção, Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121 (Candeal), Salvador, BA, Brazil;Departamento de Medicina Veterinária Preventiva e Produção Animal, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Salvador, BA, Brazil;Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT, Salvador, BA, Brazil;Laboratório de Tecnologia Diagnóstica, Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos, FIOCRUZ, Rio de Janeiro, RJ, Brazil

关键词: Leishmaniasis;    Recombinant antigens;    MAPIA;   

Others  :  1147577
DOI  :  10.1186/s13071-015-0651-6

 received in 2014-09-08, accepted in 2015-01-11,  发布年份 2015

【 摘 要 】

Background

Domestic dogs are the principal reservoir hosts of Leishmania infantum in regions where visceral leishmaniasis (VL) is endemic. Although serologic methods are frequently used for the screening of infected dogs, antibody-based tests require further assessment, due to lack of sensitivity and specificity. In this study, we employed a multi-antigen printing immunoassay (MAPIA) to compare the antibody responses to novel recombinant proteins of L. infantum with the potential for the detection of canine VL.

Findings

MAPIA strips were prepared employing 12 recombinant proteins. Antibody reactivity to these antigens was compared using a panel of sera collected from clinically asymptomatic (n = 16) and symptomatic (n = 41) culture-positive animals. Our findings showed that the canine immune response to antigen differs between dogs and depends on infection status. Using this screening assay, when five out of the 12 antigens were combined, an overall 81% detection rate of L. infantum-infected dogs was achieved.

Conclusions

We conclude that MAPIA is an effective screening tool to rapidly select multiple antigens of diagnostic utility to be used in a more sensitive point of care diagnostic test such as the Dual-Path Platform (DPP) multiplex test for the rapid detection of infected dogs.

【 授权许可】

   
2015 de Oliveira et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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