Different amounts of protein-bound citrulline and homocitrulline in foot joint tissues of a patient with anti-citrullinated protein antibody positive erosive rheumatoid arthritis

Journal of Translational Medicine

Juha Risteli³  Petri Lehenkari²  Eeva Alasaarela¹  Jukka Melkko⁵  Marja-Kaisa Koivula³  Sanna Turunen⁴ 

[1] Department of Internal Medicine, Division of Rheumatology, Oulu University Hospital, Oulu, Finland;Surgery Clinic, Oulu University Hospital, Oulu, Finland;Northern Finland Laboratory Centre NordLab, Oulu University Hospital, Oulu, Finland;Department of Clinical Chemistry, Institute of Diagnostics, University of Oulu, Oulu, Finland;Department of Pathology, Oulu University Hospital, Oulu, Finland

关键词: Carbamylation;    Tissue;    Autoantibodies;    Rheumatoid arthritis;    Homocitrulline;    Citrulline;   

Others  :  826380
DOI  :  10.1186/1479-5876-11-224

 received in 2013-05-22, accepted in 2013-09-17,  发布年份 2013

【 摘 要 】

Background

Antibodies binding to citrullinated proteins are a frequent finding in rheumatoid arthritis patients and may precede the onset of clinical symptoms several years. The antibodies are a predisposing factor for bone erosions but their origin is unknown. In this study we analyze in detail the levels of protein bound citrulline and homocitrulline in several tissue samples of a single erosive arthritic surgery patient.

Methods

Serum antibodies binding to CCP, MCV and citrulline- or homocitrulline-containing type I and II collagen carboxytelopeptides were measured. Tissue samples of a single RA patient, taken in two separate operations performed with two-year time span were hydrolyzed and analyzed for citrulline and homocitrulline content by HPLC.

Results

Protein-bound citrulline and homocitrulline were found in several joint tissues of a RA patient with ACPA-positive erosive disease. The amount of homocitrulline stayed relatively constant between the different tissues. The amount of citrulline in erosive tissue was 3-times higher than in non-erosive tissue in the first operation. In the samples of the second operation 3-4-times higher mean amounts of citrulline were found in two out of the six tissues investigated.

Conclusions

Homocitrulline is present in rheumatoid nodule together with citrulline. There is more variation in the amount of citrulline than in the amount of homocitrulline between the tissues. The tissue sample containing the most citrulline was the most erosive.

【 授权许可】

   
2013 Turunen et al.; licensee BioMed Central Ltd.

【 图 表 】

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