The different molecular forms of urine neutrophil gelatinase-associated lipocalin present in dogs with urinary diseases

BMC Veterinary Research

Ya-Jane Lee⁴  Fong-Yuan Lin³  Min-Liang Wong¹  Anais Virilli²  Guillaume Belot²  Kwong-chung Tung¹  Hsiao-Chi Chiou¹  Wei-Li Hsu³ 

[1] Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan;National Veterinary School of Toulouse, Toulouse, France;Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan;Institute of Veterinary Clinical Science, School of Veterinary Medicine, College of Bio-Resources and Agriculture, National Taiwan University, No.1, Sec.4, Roosevelt Rd, Taipei, Taiwan

关键词: NGAL;    Cystitis;    Biomarker;    Azotemia;   

Others  :  1119064
DOI  :  10.1186/s12917-014-0202-y

 received in 2014-04-03, accepted in 2014-08-18,  发布年份 2014

【 摘 要 】

Background

Neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for the early prediction of renal diseases. NGAL may exist as monomer, dimer and/or NGAL/MMP-9 complex forms in humans. In this study, the existence of various forms of NGAL in urine (uNGAL) was determined and whether these forms are related to the different urinary diseases found in dogs is further discussed.

Results

Eighty-one urine samples from dogs with different forms of renal disease (41), pyuria (19) and a number of non-renal related diseases (10), as well as healthy dogs (11), were collected. uNGAL concentrations and their molecular forms in dogs were measured by ELISA and Western blot analysis, respectively. The uNGAL concentrations of dogs with pyuria (median: 15.35 ng/mL) were significantly higher than those of the healthy control animals (median: 3.92 ng/mL) (p < 0.01), but lower than those of dogs with renal diseases (median: 23.77 ng/mL). Each NGAL molecular form could be detected in dog urine. In particular, monomer was detected more frequently in patients with renal disease than those with non-renal diseases; while the dimer form appeared in a significantly higher percentage of cases with pyuria compared to those without pyuria. The NGAL/MMP-9 complex was found to exist not only in the patients with cystitis, but also in the cases with renal injury.

Conclusion

Different molecular forms of uNGAL can indicate different origins of the urinary abnormalities. Determining the molecular forms of uNGAL present in diseased dogs may provide clinical workers with a tool that will help the early and more precise detection of different urinary diseases.

【 授权许可】

   
2014 Hsu et al.; licensee BioMed Central Ltd

【 图 表 】

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