Urine neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute canine kidney injury

BMC Veterinary Research

Wei-Li Hsu³  Hsu Kuo-Hsuan⁴  Min-Liang Wong²  Fong-Yuan Lin²  Chun-Ting Chang¹  Yi-Shan Lin¹  Yu-Yen Hu²  Ya-Jane Lee¹ 

[1] Veterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan;Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan;Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan;Division of Chest Medicine, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan

关键词: Polyclonal antibody;    ELISA;    Biomarker;    Kidney injury;    NGAL;   

Others  :  1119639
DOI  :  10.1186/1746-6148-8-248

 received in 2012-06-29, accepted in 2012-12-20,  发布年份 2012

【 摘 要 】

Background

Biomarkers for the early prediction of canine acute kidney injury (AKI) are clinically important. Recently, neutrophil gelatinase-associated lipocalin (NGAL) was found to be a sensitive biomarker for the prediction of human AKI at a very early stage and the development of AKI after surgery. However, NGAL has not yet been studied with respect to dog kidney diseases. The application of NGAL canine AKI was investigated in this study.

Results

The canine NGAL gene was successfully cloned and expressed. Polyclonal antibodies against canine NGAL were generated and used to develop an ELISA for measuring NGAL protein in serum and urine samples that were collected from 39 dogs at different time points after surgery.

AKI was defined by the standard method, namely a serum creatinine increase of greater than or equal to 26.5 μmol/L from baseline within 48 h. At 12 h after surgery, compared to the group without AKI (12 dogs), the NGAL level in the urine of seven dogs with AKI was significantly increased (median 178.4 pg/mL vs. 88.0 pg/mL), and this difference was sustained to 72 h.

Conclusion

As the increase in NGAL occurred much earlier than the increase in serum creatinine, urine NGAL seems to be able to serve as a sensitive and specific biomarker for the prediction of AKI in dogs.

【 授权许可】

   
2012 Lee et al.; licensee BioMed Central Ltd.

【 图 表 】

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