A first generation bovine BAC-based physical map

Genetics Selection Evolution

Edmond P Cribiu³  André Eggen³  Didier Boichard¹  Sead Taourit³  Mathieu Gautier³  Jean-Claude Save²  Marie-Françoise Mahé³  Anne Roig³  Laurent Schibler³ 

[1] Station de génétique quantitative appliquée, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France;Laboratoire de radiobiologie et d'étude du génome, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France;Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France

关键词: BAC;    physical map;    cattle;   

Others  :  1094352
DOI  :  10.1186/1297-9686-36-1-105

 received in 2003-06-18, accepted in 2003-10-17,  发布年份 2004

【 摘 要 】

A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

【 授权许可】

   
2004 INRA, EDP Sciences