Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome in Jiangxi province, China

Irish Veterinary Journal

Xuepeng Cai¹  Huanjie Jia¹  Haixue Zheng¹  Xuenong Luo¹  Wei Gong¹  Guohua Wu¹  Aijiang Guo¹ 

[1] State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Gansu Provincial Engineering and Technique Research Centre on Biological Detection, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping Yanchangbu Chengguan District, Lanzhou, 730046, China

关键词: Swine herds;    NSP2;    RT-PCR;    ELISA;    PRRS;    Highly pathogenic;   

Others  :  810977
DOI  :  10.1186/2046-0481-65-14

 received in 2011-12-30, accepted in 2012-06-25,  发布年份 2012

【 摘 要 】

In 2007, herds of pigs in Jiangxi Province, China experienced outbreaks of a severe form of suspected porcine reproductive and respiratory syndrome (PRRS) characterized by high fever, high morbidity and mortality in animals of different ages. 152 swine sera and 42 tissues (consisting of liver, lung, lymph node and kidney) from five herds of pigs were collected. Pigs were diagnosed as infected with a highly pathogenic form of the PRRS virus (PRRSV) based on ELISA and reverse transcriptase polymerase chain reaction (RT-PCR) results. Serological surveys indicated that 67-100% of the examined pig herds in Jiangxi Province were seropositive. 42 tissue samples were used to detect classical swine fever virus, porcine circovirus type 2 and PRRSV. Results indicated that only PRRSV was detected in 42 samples. 12 PRRSV amplified products of five herds, which consisted of two or three samples randomly selected from each herd, were used for sequencing. Subsequent nucleotide sequencing showed that the NSP2 gene had 99–99.7% nucleotide and 99.2–100% derived amino acid sequence identities among 12 tissues with that of the PRRS-JXA1 strain, deletions of 29 amino acids corresponded to positions 534–562 of the NSP2 gene sequence. These results revealed that the diseased pigs were all caused by fatal PRRSV variant. Compared with the same period in 2006, the number of positive cases from Jiangxi Province remained unchanged. These findings demonstrated that the highly pathogenic Northern American type PRRSV was still spreading in Jiangxi Province, China in 2007.

【 授权许可】

   
2012 Guo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Wensvoort G, Terpstra C, Pol JM, Ter Laak EA, Bloemraad M, De Kluyver EP, Kragten C, Van Buiten L, Den Besten A, Wagenaar F: Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 1991, 13:121-130.
• [2]Meulenberg JJ, Hulst MM, de Meijer EJ, Moonen PJM, den Besten A, De Kluyver EP, Wensvoort G, Moormann RJ: Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 1993, 206:62-72.
• [3]Forsberg R, Oleksiewicz MB, Petersen AM, Hein J, Botner A, Storgaard T: A molecular clock dates the common ancestor of European-type porcine reproductive and respiratory syndrome virus at more than 10 y before the emergence of disease. Virology 2001, 289:174-179.
• [4]Plagemann PG: Porcine reproductive and respiratory syndrome virus: Origin hypothesis. Emerg Infect Dis 2003, 9:903-908.
• [5]Gao ZQ, Guo X, Yang HC: Genomic characterization of two Chinese isolates of porcine respiratory and reproductive syndrome virus. Arch Virol 2004, 149:1341-1351.
• [6]Ropp SL, Wees CE, Fang Y, Nelson EA, Rossow KD, Bien M, Arndt B, Preszler S, Steen P, Christopher-Hennings J, Collins JE, Benfield DA, Faaberg KS: Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol 2004, 78:3684-3703.
• [7]Thanawongnuwech R, Amonsin A, Tatsanakit A, Damrongwatanapokin S: Genetics and geographical variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand. Vet Microbiol 2004, 101:9-21.
• [8]Meng XJ, Paul PS, Halbur PG, Lum MA: Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): Implication for the existence of two genotypes of PRRSV in the U.S.A. and Europe. Arch Virol 1995, 140:745-755.
• [9]Tian KG, Yu XL, Zhao TZ, Feng YJ, Cao Z, Wang CB, Hu Y, Chen XZ, Hu DM, Tian XS, Liu D, Zhang S, Deng XY, Ding YQ, Yang L, Zhang YX, Xiao HX, Qiao MM, Wang B, Hou LL, Wang XY, Yang XY, Kang LP, Sun M, Jin P, Wang SJ, Kitamura Y, Yan JH, Gao GF: Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS One 2007, 2(6):e526.
• [10]Meng XJ: Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Vet Microbiol 2000, 74:309-329.
• [11]Pesente P, Rebonato V, Sandri G, Giovanardi D, Ruffoni LS, Torriani S: Phylogenetic analysis of ORF5 and ORF7 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) from PRRS-positive Italian farms: a showcase for PRRSV epidemiology and its consequences on farm management. Vet Microbiol 2006, 114:214-224.
• [12]Yoshii M, Kaku Y, Murakami Y, Shimizu M, Kato K, Ikeda H: Genetic variation and geographic distribution of porcine reproductive and respiratory syndrome virus in Japan. Arch Virol 2005, 150:2313-2324.
• [13]Yuan S, Murtaugh MP, Schumann FA, Mickelson D, Faaberg KS: Characterization of heteroclite subgenomic RNAs associated with PRRSV infection. Virus Res 2004, 105:75-87.
• [14]Chen J, Liu T, Zhu CG, Jin YF, Zhang YZ: Genetic variation of Chinese PRRSV strains based on ORF5 sequence. Biochem Genet 2006, 44:425-435.
• [15]An TQ, Zhou YJ, Liu GQ, Tian ZJ, Li J, Qui HJ, Tong GZ: Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: coexistence of two NA-subgenotypes with great diversity. Vet Microbiol 2007, 123:43-52.
• [16]Allende R, Lewis TL, Lu Z, Rock DL, Kutish GF, Ali A, Doster AR, Osorio FA: North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. J Gen Virol 1999, 80:307-315.
• [17]Shen S, Kwang J, Liu W, Liu DX: Determination of the complete nucleotide sequence of a vaccine strain of porcine reproductive and respiratory syndrome virus and identification of the Nsp2 gene with a unique insertion. Arch Virol 2000, 145:871-883.
• [18]Fang Y, Kim DY, Ropp S, Steen P, Christopher-Hennings J, Nelson EA, Rowland RR: Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res 2004, 100:229-235.
• [19]Feng YJ, Zhao TZ, Nguyen T, Inui K, Ma Y, Nguyen TH, Nguyen VC, Liu D, Bui QA, To LT, Wang CB, Tian KG, Gao GF: Porcine Respiratory and Reproductive Syndrome Virus Variants, Vietnam and China, 2007. Emerg Infect Dis 2008, 14:1774-1776.
• [20]Hu HX, Li XL, Zhang ZF, Shuai JB, Chen N, Liu GQ, Fang WH: Porcine reproductive and respiratory syndrome viruses predominant in southeastern China from 2004 to 2007 were from a common source and underwent further divergence. Arch Virol 2009, 154:391-398.
• [21]Song J, Shen D, Cui J, Zhao BH: Accelerated evolution of PRRSV during recent outbreaks in China. Virus Genes 2010, 41:241-245.
• [22]Tong GZ, Zhou YJ, Hao XF, Tian ZJ, An TQ, Qiu HJ: Highly pathogenic porcine reproductive and respiratory syndrome, China. Emerg Infect Dis 2007, 13:1434-1436.
• [23]Liu SS, Zhao YR, Hu QB, Lv CC, Zhang CF, Zhao R, Hu F, Lin WC, Cui SJ: A multiplex RT-PCR for rapid and simultaneous detection of porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. J Virol Methods 2011, 172:88-92.
• [24]Qiao J, Chen C, feng G, Guizheng J: Detection of classical swine fever virus by reverse transcription polymerase chain reaction. Journal of Tarim University of Agricultural Reclamation 2001, 13:25-27.
• [25]Wang X, Liu X, Liang M, Zheng Y, Liu L: Detection of Porcine Circovirus Type 2 by PCR Technique. Chinese Agricultural Science Bulletin 2007, 9:28-31.
• [26]Li YF, Wang XL, Bo KT, Wang XW, Tang B, Yang BS, Jiang WN, Jiang P: Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China. Vet J 2007, 174:577-584.