Human adipose-derived stem cells cultured in keratinocyte serum free medium: Donor’s age does not affect the proliferation and differentiation capacities

Journal of Biomedical Science

Tang-Yuan Chu³  Hwan-Wun Liu¹  Wei-Ting Hung²  Hsiang-Lan Chou²  Dah-Ching Ding² 

[1] Department of Occupational Medicine, Buddhist Tzu Chi General Hospital, Tzu Chi University, Hualien, Taiwan;Stem Cell Laboratory, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan;Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan

关键词: Telomere length;    Differentiation;    Proliferation;    Donor’s age;    Adipose-derived stem cells;   

Others  :  823402
DOI  :  10.1186/1423-0127-20-59

 received in 2013-06-03, accepted in 2013-08-05,  发布年份 2013

【 摘 要 】

Background

Although donor age-related effects of characteristics of mesenchymal stem cells (MSC), such as a decrease in the proliferation and differentiation capacity and an increase of senescence and apoptosis, are evident, such effects are generally less prominent in adipose-derived stem cells (ASC). Using a hormone and growth factor rich medium (KFSM), this study cultured ASC from abdominal subcutaneous fat of 27 adult females in three age groups: 30-39 y, 40-49 y and 50-60 y, and investigated the growth and differentiation characteristics.

Results

The derived ASC had an immunophenotype similar to that of bone marrow derived MSC (BMSC). They could be stably expanded with an average population doubling time of 21.5 ± 2.3 h. Other than a higher pre-adipogenic commitment and a lower adipogenic differentiation capability in ASC derived from the old age group, other characteristics including proliferation rate, doubling time, telomere length, as well as the osteogenic and chondrogenic differentiation capacity were the same regardless of the donor’s age.

Conclusions

The study demonstrates a promising proliferation and differentiation capabilities of ASC regardless of the donor’s age. The compromised adipogenic potential in the older donors could be a benefit for their application in regeneration therapy.

【 授权许可】

   
2013 Ding et al.; licensee BioMed Central Ltd.

【 图 表 】

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