Molecular profile of cochlear immunity in the resident cells of the organ of Corti

Journal of Neuroinflammation

Bo Hua Hu²  Youyi Dong²  Daniel Cartwright²  Jennifer Jamison³  Jonathan Bard³  Shuzhi Yang¹  R Robert Vethanayagam²  Qunfeng Cai² 

[1] Department of Otolaryngology, The first affiliated Hospital to Chinese PLA General Hospital, 51, Fucheng Road, Haidian District, Beijing 100048, China;Center for Hearing and Deafness, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo 14214, NY, USA;Next-Generation Sequencing and Expression Analysis Core, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo, 701 Ellicott Street, Buffalo 14260, NY, USA

关键词: Supporting cells;    Inflammation;    Sensory cells;    Noise;    The organ of Corti;    Immunity;   

Others  :  1150490
DOI  :  10.1186/s12974-014-0173-8

 received in 2014-07-09, accepted in 2014-09-25,  发布年份 2014

【 摘 要 】

Background

The cochlea is the sensory organ of hearing. In the cochlea, the organ of Corti houses sensory cells that are susceptible to pathological insults. While the organ of Corti lacks immune cells, it does have the capacity for immune activity. We hypothesized that resident cells in the organ of Corti were responsible for the stress-induced immune response of the organ of Corti. This study profiled the molecular composition of the immune system in the organ of Corti and examined the immune response of non-immune epithelial cells to acoustic overstimulation.

Methods

Using high-throughput RNA-sequencing and qRT-PCR arrays, we identified immune- and inflammation-related genes in both the cochlear sensory epithelium and the organ of Corti. Using bioinformatics analyses, we cataloged the immune genes expressed. We then examined the response of these genes to acoustic overstimulation and determined how changes in immune gene expression were related to sensory cell damage.

Results

The RNA-sequencing analysis reveals robust expression of immune-related genes in the cochlear sensory epithelium. The qRT-PCR array analysis confirms that many of these genes are constitutively expressed in the resident cells of the organ of Corti. Bioinformatics analyses reveal that the genes expressed are linked to the Toll-like receptor signaling pathway. We demonstrate that expression of Toll-like receptor signaling genes is predominantly from the supporting cells in the organ of Corti cells. Importantly, our data demonstrate that these Toll-like receptor pathway genes are able to respond to acoustic trauma and that their expression changes are associated with sensory cell damage.

Conclusion

The cochlear resident cells in the organ of Corti have immune capacity and participate in the cochlear immune response to acoustic overstimulation.

【 授权许可】

   
2014 Cai et al.; licensee BioMed Central Ltd.

【 图 表 】

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