Genome sequencing of multidrug resistant novel Clostridium sp. BL8 reveals its potential for pathogenicity

Gut Pathogens

Yogesh S Shouche³  Dilip R Ranade¹  Mandar Hemant Rasane³  Vikram B Lanjekar¹  Sudarshan Anand Shetty³  Nachiket Prakash Marathe² 

[1] Agharkar Research Institute, Gopal Ganesh Agarkar Road, Pune 411004, Maharashtra, India;Current Address: Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy at the University of Gothenburg, Guldhedsgatan 10, SE-413 46, Göteborg, Sweden;Microbial Culture Collection, National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune 411 007, Maharashtra, India

关键词: Ion Torrent PGM^™;    Antibiotic resistance;    Virulence;    Clostridium;   

Others  :  1133844
DOI  :  10.1186/1757-4749-6-30

 received in 2014-05-21, accepted in 2014-07-13,  发布年份 2014

【 摘 要 】

Background

The human gut microbiome is important for maintaining the health status of the host. Clostridia are key members of the human gut microbiome, carrying out several important functions in the gut environment. Hence understanding the role of different Clostridium species isolated from human gut is essential. The present study was aimed at investigating the role of novel Clostridium sp. isolate BL8 in human gut using genome sequencing as a tool.

Findings

The genome analysis of Clostridium sp. BL8 showed the presence of several adaptive features like bile resistance, presence of sensory and regulatory systems, presence of oxidative stress managing systems and presence of membrane transport systems. The genome of Clostridium sp. BL8 consists of a wide variety of virulence factors like phospholipase C (alpha toxin), hemolysin, aureolysin and exfoliative toxin A, as well as adhesion factors, proteases, Type IV secretion system and antibiotic resistance genes. In vitro antibiotic sensitivity testing showed that Clostridium sp. BL8 was resistant to 11 different tested antibiotics belonging to 6 different classes. The cell cytotoxicity assay confirmed the cytotoxic effect of Clostridium sp. BL8 cells, which killed 40% of the Vero cells after 4 hrs of incubation.

Conclusions

Clostridium sp. BL8 has adapted for survival in human gut environment, with presence of different adaptive features. The presence of several virulence factors and cell cytotoxic activity indicate that Clostridium sp. BL8 has a potential to cause infections in humans, however further in vivo studies are necessary to ascertain this fact.

【 授权许可】

   
2014 Marathe et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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