Innate and adaptive immune responses of snatch-farrowed porcine-colostrum-deprived pigs to Mycoplasma hyopneumoniae vaccination

BMC Veterinary Research

John CS Harding²  Deborah M Haines¹  Carolyn Ashley²  Andrea Ladinig²  Yanyun Huang³ 

[1] Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon S7N 5B, SK, Canada;Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon S7N 5B, SK, Canada;Prairie Diagnostic Services Inc., Saskatoon, SK, Canada

关键词: Fluorescent microsphere immunoassay;    Immunity;    Cytokine;    Mycoplasma hyopneumoniae;    Animal model;    Snatch-farrowed porcine-colostrum-deprived pigs;   

Others  :  1118982
DOI  :  10.1186/s12917-014-0219-2

 received in 2014-05-14, accepted in 2014-09-17,  发布年份 2014

【 摘 要 】

Background

The snatch-farrowed porcine-colostrum-deprived (SF-pCD) pig model, in which neonates are raised on commercially available bovine colostrum, is an alternative model for porcine infectious disease research. It is not known if SF-pCD pigs possess growth performance and immunity comparable to conventional, farm-raised pigs. The current experiment compared growth performance and immune responses of SF-pCD pigs to their farm-raised siblings following Mycoplasma hyopneumoniae (Mhyo) vaccination. Twelve SF-pCD and 13 farm-raised siblings were vaccinated on day 7 (D7) and D26 of age. Body weights were measured once or twice weekly and average daily gain (ADG) was calculated. Peripheral blood mononuclear cells (PBMC) were isolated on D40. Cytokine secretion from PBMC stimulated with Mhyo antigen or phorbol myristate acetate plus ionomycin (PMA/Iono) was assessed using a multiplexed fluorescent microsphere immunoassay (FMIA). Additionally, interferon gamma (IFNγ) secretion from stimulated PBMC was assessed using ELISPOT. Mhyo IgG titers were measured by an ELISA in D40 sera.

Results

Growth performance did not differ between groups before weaning, but SF-pCD pigs had higher ADG after weaning. In response to Mhyo stimulation, numbers of IFNγ secreting PBMC and levels of interleukin 8 (IL8) and IL10 in PBMC supernatants were significantly higher in SF-pCD pigs, as were Mhyo antibody levels in sera, and levels of IL1β, IL8 and IL12 in supernatants of PMA/Iono stimulated PBMC.

Conclusions

Under the conditions of this experiment, SF-pCD pigs demonstrated superior growth performance and enhanced humoral and cell-mediated immunity following vaccination. Whether or not this reflects greater resistance or tolerance to infection is unknown but the ability to react positively to the vaccination provides evidence that SF-pCD pigs are a suitable alternative model for swine disease research.

【 授权许可】

   
2014 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Butler JE, Weber P, Sinkora M, Baker D, Schoenherr A, Mayer B, Francis D: Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-dependent and type 2 T-independent antigens. J Immunol 2002, 169(12):6822-6830.
• [2]Blanco I, Galina-Pantoja L, Oliveira S, Pijoan C, Sanchez C, Canals A: Comparison between Haemophilus parasuis infection in colostrum-deprived and sow-reared piglets. Vet Microbiol 2004, 103(1/2):21-27.
• [3]Oliveira S, Galina L, Blanco I, Canals A, Pijoan C: Naturally-farrowed, artificially-reared pigs as an alternative model for experimental infection by Haemophilus parasuis. Can J Vet Res 2003, 67(2):146.
• [4]Huang Y, Haines DM, Harding JCS: Snatch-farrowed, porcine-colostrum-deprived (SF-pCD) pigs as a model for swine infectious disease research. Can J Vet Res 2013, 77(2):81-88.
• [5]Lawson S, Lunney J, Zuckermann F, Osorio F, Nelson E, Welbon C, Clement T, Fang Y, Wong S, Kulas K: Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination. Vaccine 2010, 28(32):5356-5364.
• [6]Puppe B, Tuchscherer A: The development of suckling frequency in pigs from birth to weaning of their piglets: a sociobiological approach. Anim Sci 2000, 71(2):273-280.
• [7]Berg-Brown NN, Gronski MA, Jones RG, Elford AR, Deenick EK, Odermatt B, Littman DR, Ohashi PS: PKCθ signals activation versus tolerance in vivo. J Exp Med 2004, 199(6):743-752.
• [8]Harris W, Gollapudi S, Gupta S: Anti-CD3-induced changes in protein kinase C isozymes expression in human CD4+ and CD8+ T lymphocytes. J Clin Immunol 1995, 15(5):232-241.
• [9]Tan SL, Parker PJ: Emerging and diverse roles of protein kinase C in immune cell signalling. Biochem J 2003, 376(3):545-552.
• [10]Murtaugh MP, Baarsch MJ, Zhou Y, Scamurra RW, Lin G: Inflammatory cytokines in animal health and disease. Vet Immunol Immunopathol 1996, 54(1–4):45-55.
• [11]Murphy KP: Janeway’s Immunobiology. Garland Science, Taylor & Francis Group, LLC., New York, USA; 2012.
• [12]Moore KW, de Waal MR, Coffman RL, O’Garra A: Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 2001, 19:683-765.
• [13]Murtaugh MP, Johnson CR, Xiao ZG, Scamurra RW, Zhou YL: Species specialization in cytokine biology: is interleukin-4 central to the T H1-T H2 paradigm in swine? Dev Comp Immunol 2009, 33(3):344-352.
• [14]Björkstén B, Sepp E, Julge K, Voor T, Mikelsaar M: Allergy development and the intestinal microflora during the first year of life. J Allergy Clin Immunol 2001, 108(4):516-520.
• [15]Inman C, Haverson K, Konstantinov S, Jones P, Harris C, Smidt H, Miller B, Bailey M, Stokes C: Rearing environment affects development of the immune system in neonates. Clin Exp Immunol 2010, 160(3):431-439.
• [16]Funderburke D, Seerley R: The effects of postweaning stressors on pig weight change, blood, liver and digestive tract characteristics. J Anim Sci 1990, 68(1):155-162.
• [17]Gallagher DP, Cotter PF, Mulvihill DM: Porcine milk proteins: a review. Int Dairy J 1997, 7(2–3):99-118.
• [18]Boudry C, Dehoux JP, Wavreille J, Portetelle D, Thewis A, Buldgen A: Effect of a bovine colostrum whey supplementation on growth performance, faecal Escherichia coli population and systemic immune response of piglets at weaning. Animal 2008, 2(5):730-737.
• [19]Boudry C, Dehoux J-P, Portetelle D, Buldgen A: Bovine colostrum as a natural growth promoter for newly weaned piglets: a review. Biotechnol Agron Soc Environ 2008, 12(2):157-170.
• [20]Steenhard NR, Jungersen G, Kokotovic B, Beshah E, Dawson HD, Urban JF Jr, Roepstorff A, Thamsborg SM: Ascaris suum infection negatively affects the response to a Mycoplasma hyopneumoniae vaccination and subsequent challenge infection in pigs. Vaccine 2009, 27(37):5161-5169.