Enzymatic cleavage of histone H3: a new consideration when measuring histone modifications in human samples

Clinical Epigenetics

Mary V Gamble¹  Caitlin G Howe² 

[1] Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 W. 168th Street, Room 1107E, New York 10032, NY, USA;Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 650 W. 168th Street, Room 1618, New York 10032, NY, USA

关键词: Histone modifications;    Enzymatic cleavage;    Histone H3;   

Others  :  1092750
DOI  :  10.1186/s13148-014-0041-5

 received in 2014-11-25, accepted in 2014-12-22,  发布年份 2015

【 摘 要 】

Histone modifications are increasingly being used as biomarkers of cancer prognosis and survival. However, we identified a cleavage product of histone H3 in human peripheral blood mononuclear cells, which interferes with measures of certain H3 modifications. Therefore, the potential for enzymatic cleavage of histones should be considered when measuring histone modifications in human samples. Furthermore, the enzymatic cleavage of human H3 is itself a fascinating area of research and two important questions remain to be answered: 1) Does cleavage of human H3 occur in vivo, as it does in other organisms? and 2) Does it serve a biologically important function?

【 授权许可】

   
2015 Howe and Gamble; licensee Biomed Central.

【 图 表 】

Figure 1.

Figure 2.

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