Saturated fatty acid palmitate-induced insulin resistance is accompanied with myotube loss and the impaired expression of health benefit myokine genes in C2C12 myotubes

Lipids in Health and Disease

Hengyi Xiao¹  Zhe Wang¹  Xiaojuan Han¹  Xu Wang¹  Jie Zhang¹  Chunfen Mo¹  Dandan Wei¹  Ming Yang¹ 

[1] Department of Geriatrics, Regenerative Medicine Research Center, Laboratory of Stem cell biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Keyuan 4-1, Gaopeng Street, High-tech Zone, Chengdu 610041, China

关键词: Insulin resistance;    Myokine;    Myotube loss;    Palmitate;   

Others  :  1160021
DOI  :  10.1186/1476-511X-12-104

 received in 2013-05-01, accepted in 2013-07-02,  发布年份 2013

【 摘 要 】

Background

Excessive circular fatty acid, particlarly saturated fatty acid, can result in insulin resistance in skeletal muscle, but other adverse effects of fatty acid accumulation in myocytes remain unclear.

Methods

Differentiated C2C12 myotubes were used. The effects of palmitate on cell viability, glucose uptake, gene expression and myotube loss were evaluated by MTT assay, 2NBDG uptake, qRT-PCR, Western Blot and crystal staining-based myotube counting, respectively. In some expreiments, oleate was administrated, or the inhibitors of signaling pathways were applied.

Results

Palmitate-induced cellular insulin resistance was clarified by the reduced Akt phosphorylation, glucose uptake and Glut4 expression. Palmitate-caused myotube loss was clearly observed under microscope and proved by myotube counting and expression analysis of myotube marker genes. Moreover, palmitate-induced transcriptional suppression of three health benefit myokine genes (FNDC5, CTRP15 and FGF21) was found, and the different involvement of p38 and PI3K in the transcription of these genes was noticed.

Conclusions

Palmitate-induced insulin resistance accompanys myotube loss and the impaired expression of FNDC5, CTRP15 and FGF21genes in C2C12 myotubes. These results provide novel evidence indicating the negative role of high concentration of palmitate in myotubes.

【 授权许可】

   
2013 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

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