Highland cattle and Radix labiata, the hosts of Fascioloides magna

BMC Veterinary Research

Martin Kašný²  Petr Horák¹  Kristýna Vrbová¹  Jan Pankrác¹  Klára Melounová¹  Veronika Siegelová¹  Monika Košťáková¹  Roman Leontovyč¹ 

[1] Faculty of Science, Department of Parasitology, Charles University in Prague, Viničná 7, Prague 128 44, Czech Republic;Faculty of Science, Department of Botany and Zoology, Masaryk University, Kotlářská 2, Brno 611 37, Czech Republic

关键词: Pseudocyst;    Histology;    ITS2;    Molecular determination;    Highland cattle;    Galba truncatula;    Radix labiata;    Fasciola hepatica;    Fascioloides magna;   

Others  :  1119329
DOI  :  10.1186/1746-6148-10-41

 received in 2013-07-03, accepted in 2014-01-28,  发布年份 2014

【 摘 要 】

Background

Fascioloides magna is a pathogenic fluke introduced to Europe ca 140 years ago. As it is spreading over the continent, new intermediate and definitive hosts might be involved in transmission of the parasite. In Europe, several studies reported potential new intermediate snail hosts (Radix spp.) for F. magna, and also several cases of fascioloidosis of wild and domestic animals were published. However, the data based on molecular and histological analyses confirming these findings remained unreported. This study aims to refer to unique findings of F. magna in European snails and domestic animals (the first observation in the Czech Republic in the last 30 years) and demonstrate the use of molecular techniques in determination of F. magna.

Results

Two snails of R. labiata naturally infected with F. magna were found; mature cercariae and daughter rediae were observed. Maturity of cercariae was checked by histological methods, however, their ability to encyst was not confirmed. Co-infection of F. magna and Fasciola hepatica in the liver of two highland cattle bulls was proved. Adult fasciolid flukes producing eggs were found in the liver pseudocysts (F. magna) and the bile ducts (F. hepatica). Identification of intermediate hosts, intramolluscan stages, adult flukes and eggs was performed by sequencing the ITS2 region. Connection of F. magna pseudocysts with the gut (via the bile ducts) was not confirmed by means of histological and coprological examinations.

Conclusions

For the first time, Radix labiata was confirmed as the snail host for F. magna under natural conditions and, together with the finding of F. magna infection in cattle, we can expect further transmission of F. magna from wildlife to livestock in localities shared by these hosts.

【 授权许可】

   
2014 Leontovyč et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Erhardová-Kotrlá B: The Occurence of Fascioloides magna (Bassi, 1875) in Czechoslovakia. Prague: Academia; 1971:1-124.
• [2]Bassi R: Sulla cachessia ittero-verminosa, o marciaia, causata dal Distomum magnum. Medico Vet Torino 1875, (11-12):497-515. S.4, v.4
• [3]Kašný M, Beran L, Siegelová V, Siegel T, Leontovyč R, Beránková K, Pankrác J, Košťáková M, Horák P: Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Vet Med (Praha) 2012, 57:101-109.
• [4]Bazsalovicsová E, Král’ová-Hromadová I, Špakulová M, Reblánová M, Oberhauserová K: Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia 2010, 47:76-82.
• [5]Král’ová-Hromadová I, Bazsalovicsová E, Štefka J, Špakulová M, Vávrová S, Szemes T, Tkach V, Trudgett A, Pybus M: Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. Int J Parasitol 2011, 41:373-383.
• [6]Ursprung J, Joachim A, Prosl H: Incidence and control of the American giant liver fluke, Fascioloides magna, in a population of wild ungulates in the Danubian wetlands east of Vienna. Berl Münch Tier Woch 2006, 119:316-323.
• [7]Majoros G, Stojkov V: Appearance of the large American liver fluke Fascioloides magna (Bassi, 1875) (Trematoda: Fasciolata) in Hungary. Parasitol Hung 1994, 28:27-38.
• [8]Rajkovic-Janje R, Bosnic S, Rimac D, Gojmerac T: The prevalence of American liver fluke Fascioloides magna (Bassi 1875) in red deer from Croatian hunting grounds. Eur J Wildlife Res 2008, 54:525-528.
• [9]Balbo T, Lanfranchi P, Rossi L, Meneguz P: Health management of a red deer population infected by Fasciololoides magna (Bassi, 1875) Ward, 1917 (1). Ann Fac Med Vet Torino 1987, 32:23-33.
• [10]Sinistin DF: The life histories of some American liver flukes. Z Parasiten 1933, 6:170-191.
• [11]Swales WE: The life cycle of Fascioloides magna (Bassi, 1875), the large liver fluke of ruminants in Canada with observations on the bionomics of the larval stages and the intermediate hosts, pathology of Fascioloidiasis magna, and control measures. Can J Res 1935, 12:177-215.
• [12]Griffiths HJ: Stagnicola (Hinckleyia) caperata (Say), a Natural Intermediate Host for Fascioloides magna (Bassi, 1875), in Minnesota. J Parasitol 1959, 45:146.
• [13]Laursen JR, Stromberg BE: Fascioloides magna intermediate snail hosts: habitat preferences and infection parameters. J Parasitol 1993, 79:302.
• [14]Haider M, Hörweg C, Liesinger K, Sattmann H, Walochnik J: Recovery of Fascioloides magna (Digenea) population in spite of treatment programme? Screening of Galba truncatula (Gastropoda, Lymnaeidae) from Lower Austria. Vet Parasitol 2012, 187:445-451.
• [15]Schniebs K, Glöer P, Vinarski MV, Hundsdoerfer AK: Intraspecific morphological and genetic variability in the European freshwater snail Radix labiata (Rossmaessler, 1835) (Gastropoda : Basommatophora: Lymnaeidae). Contrib Zool 2013, 82:55-68.
• [16]Faltýnková A, Horáčková E, Hirtová L, Novobilský A, Modrý D, Scholz T: Is Radix peregra a new intermediate host of Fascioloides magna (Trematoda) in Europe? Field and experimental evidence. Acta Parasitol 2006, 51:87-90.
• [17]Chroustová E, Hůlka J, Jaroš J: 1980 Chroustová - Prevalence a terapie fascioloidózy skotu bithionosulfoxidem. Vet Med (Praha) 1980, 25:557-563.
• [18]Schniebs K, Glöer P, Vinarski MV, Hundsdoerfer AK: Intraspecific morphological and genetic variability in Radix balthica (Linnaeus 1758) (Gastropoda: Basommatophora: Lymnaeidae) with morphological comparison to other European Radix species. J Conchol 2011, 40:657-678.
• [19]Griffiths HJ: Galba modicella and Lymnaea stagnalis as experimental intermediate hosts for Fascioloides magna in Minnesota. J Parasitol 1973, 59:121.
• [20]Rondelaud D, Novobilský A, Vignoles P, Treuil P, Koudela B, Dreyfuss G: First studies on the susceptibility of Omphiscola glabra (Gastropoda: Lymnaeidae) from central France to Fascioloides magna. Parasitol Res 2006, 98:299-303.
• [21]Horsák M, Dvořák L, Juřičková L: Greenhouse gastropods of the Czech Republic: current stage of research. Malacol News 2004, 22:141-147.
• [22]Pointier JP, Coustau C, Rondelaud D, Theron A: Pseudosuccinea columella (Say 1817) (Gastropoda, Lymnaeidae), snail host of Fasciola hepatica: first record for France in the wild. Parasitol Res 2007, 101:1389-1392.
• [23]Foreyt WJ, Todd AC: Development of the large American liver fluke, Fascioloides magna, in whitetailed deer, cattle, and sheep. J Parasitol 1976, 62:26-32.
• [24]Pybus MJ: Liver flukes. In Parasitic Diseases in Wild Mammals. Iowa: Iowa State University Press; 2001:121-149.
• [25]Foreyt WJ, Todd AC: The occurrence of Fascioloides magna and Fasciola hepatica together in the livers of naturally infected cattle in south Texas, and the incidence of the flukes in cattle, white-tailed deer, and feral hogs. J Parasitol 1972, 58:1010-1011.
• [26]Lankester MV: Parelaphostrongylus tenis (Nematoda) and Fascioloides magna (Trematoda) in moose of southeastern Manitoba. Can J Zool 1974, 52:235-239.
• [27]Naem S, Budke CM, Craig TM: Morphological characterization of adult Fascioloides magna (Trematoda: Fasciolidae): first SEM report. Parasitol Res 2012, 110:971-978.
• [28]Conboy GA, Stromberg BE: Hematology and clinical pathology of experimental Fascioloides magna infection in cattle and guinea pigs. Vet Parasitol 1991, 40:241-255.
• [29]Huňová K, Kašný M, Hampl V, Leontovyč R, Kuběna A, Mikeš L, Horák P: Radix spp.: identification of trematode intermediate hosts in the Czech Republic. Acta Parasitol 2012, 57:273-284.
• [30]Wilson RA, Denison J: The parasitic castration and gigantism of Lymnaea truncatula infected with the larval stages of Fasciola hepatica. Parasitol Res 1980, 61:109-119.
• [31]Anh NT, Phuong NT, Ha GH, Thu LT, Johansen MV, Murrell DK, Thamsborg SM: Evaluation of techniques for detection of small trematode eggs in faeces of domestic animals. Vet Parasitol 2008, 156:346-349.
• [32]Almeyda-Artigas RJ, Bargues MD, Mas-Coma S: ITS-2 rDNA sequencing of Gnathostoma species (Nematoda) and elucidation of the species causing human gnathostomiasis in the Americas. J Parasitol 2000, 86:537-544.
• [33]Král’ová-Hromadová I, Špakulová M, Horáčková E, Turceková L, Novobilský A, Beck R, Koudela B, Marinculić A, Rajský D, Pybus M: Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. J Parasitol 2008, 94:58-67.
• [34]Harmon AF, Zarlenga DS, Hildreth MB: Improved methods for isolating DNA from Ostertagia ostertagi eggs in cattle feces. Vet Parasitol 2006, 135:297-302.
• [35]Ložek V: Klíč Československých Měkkýšů. Bratislava. Vydavateľstvo Slovenskej akadémie vied; 1956:437.
• [36]Jackewicz M: Blotniarki Europy (Gastropoda: Pulmonata: Lymnaeidae). Poznań, Poland: Wydawnictwo Kontekst; 2000:115.
• [37]Relf V, Good B, McCarthy E, Waal T: Evidence of Fasciola hepatica infection in Radix peregra and a mollusc of the family Succineidae in Ireland. Vet Parasitol 2009, 163:152-155.
• [38]Caron Y, Lasri S, Losson B: Fasciola hepatica: an assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in Belgium. Vet Parasitol 2007, 149:95-103.
• [39]Dixon KE: A morphological and histochemical study of the cystogenic cells of the cercaria of Fasciola hepatica L. Parasitology 1966, 56:287-297.
• [40]Špakulová M, Rajský D, Vodňanský J, Sokol J: Giant liver fluke (Fascioloides magna), an important liver parasite of ruminants. Bratislava: Parpress; 2003:61.
• [41]Foreyt WJ, Todd AC: Efficacy of rafoxanide and oxyclozanide against Fascioloides magna in naturally infected cattle. Am J Vet Res 1974, 35:375-377.
• [42]Simesen MG, Nielsen K, Nansen P: Some effects of experimental Fasciola hepatica infection in cattle on the serum activities of gamma-glutamyltranspeptidase and glutamic oxaloacetic transaminase. Res Vet Sci 1973, 15:32-36.
• [43]Anderson PH, Berrett S, Brush PJ, Hebert CN, Parfitt JW, Patterson DJ: Biochemical indicators of liver injury in calves with experimental fascioliasis. Vet Rec 1977, 100:43-45.
• [44]Anderson PH, Berrett S, Patterson DJ: Resistance to Fasciola hepatica in cattle II. Biochemical and morphological observations. J Comp Pathol 1978, 88:245-251.
• [45]Zmuda K, Chroust K: Motoličnatost skotu v okrese Frýdek-Místek. Veterinarstvi 2001, 51:181-183.