Confirmation that “Brachyspira hampsonii” clade I (Canadian strain 30599) causes mucohemorrhagic diarrhea and colitis in experimentally infected pigs

BMC Veterinary Research

John C S Harding²  Joseph E Rubin³  Susan E Detmer¹  Hollie D Lemieux²  Champika Fernando³  Janet E Hill³  Matheus O Costa³ 

[1] Department of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada;Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, SK, Canada;Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Dr., S7N 5B4 Saskatoon, SK, Canada

关键词: Porcine;    Pig;    Colitis;    Diarrhea;    Bloody;    Mucohemorrhagic;    Swine dysentery;    “Brachyspira hampsonii”;   

Others  :  866768
DOI  :  10.1186/1746-6148-10-129

 received in 2014-01-26, accepted in 2014-05-28,  发布年份 2014

【 摘 要 】

Background

“Brachyspira hampsonii”, discovered in North America in 2010 associated with dysentery-like illness, is an economically relevant swine pathogen resulting in decreased feed efficiency and increased morbidity, mortality and medication usage. “B. hampsonii” clade II strain 30446 has been shown to be causally associated with mucohemorrhagic diarrhea and colitis. Our objectives were to determine if “Brachyspira hampsonii” clade I strain 30599 is pathogenic to pigs, and to evaluate the relative diagnostic performance of three ante mortem sampling methodologies (direct PCR on feces, PCR on rectal GenoTube Livestock swabs, Brachyspira culture from rectal swabs). Five-week old pigs were intragastrically inoculated thrice with 10⁸ genomic equivalents "B. hampsonii" (n = 12), or served as sham controls (n = 6). Feces were sampled and consistency assessed daily. Necropsies were performed 24 h after peak clinical signs.

Results

One pig died due to unrelated illness. Nine of 11 inoculated pigs, but no controls, developed mucoid or mucohemorrhagic diarrhea (MHD). Characteristic lesions of swine dysentery were observed in large intestine. “B. hampsonii” strain 30599 DNA was detected by qPCR in feces of all inoculated pigs for up to 6 days prior to the onset of MHD. The organism was isolated from the feces and colons of pigs demonstrating MHD, but not from controls. B. intermedia was isolated from inoculated pigs without MHD, and from 5 of 6 controls.

Conclusions

We conclude that “Brachyspira hampsonii” clade I strain 30599 is pathogenic and causes mucohemorrhagic diarrhea and colitis in susceptible pigs. Moreover, the three sampling methodologies performed similarly. GenoTube Livestock, a forensic swab designed to preserve DNA during shipping is a useful tool especially in settings where timely transport of diagnostic samples is challenging.

【 授权许可】

   
2014 Costa et al.; licensee BioMed Central Ltd.

【 图 表 】

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