Pathological alterations in the gastrointestinal tract of a porcine model of DMD

Cell & Bioscience

卷:11

Renzhi Han¹  Hongsheng Ouyang²  Daxin Pang²  Xiaodong Zou²  Xiaochun Tang² 

[1] Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, The Ohio State University Wexner Medical Center;

[2] Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University;

关键词: CRISPR;    Duchenne muscular dystrophy;    Genome editing;    Gastrointestinal tract;    Pig;    Porcine;   

DOI  :  10.1186/s13578-021-00647-9

来源: DOAJ

【 摘 要 】

Abstract Background Patients with Duchenne muscular dystrophy (DMD) develop severe skeletal and cardiac muscle pathologies, which result in premature death. Therefore, the current therapeutic efforts are mainly targeted to correct dystrophin expression in skeletal muscle and heart. However, it was reported that DMD patients may also exhibit gastrointestinal and nutritional problems. How the pathological alterations in gastrointestinal tissues contribute to the disease are not fully explored. Results Here we employed the CRISPR/Cas9 system combined with somatic nuclear transfer technology (SCNT) to establish a porcine model of DMD and explored their pathological alterations. We found that genetic disruption of dystrophin expression led to morphological gastrointestinal tract alterations, weakened the gastrointestinal tract digestion and absorption capacity, and eventually led to malnutrition and gastric dysfunction in the DMD pigs. Conclusions This work provides important insights into the pathogenesis of DMD and highlights the need to consider the gastrointestinal dysfunction as an additional therapeutic target for DMD patients.

【 授权许可】

Unknown